Identification of Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus by rDNA-based techniques

• Published in: Systematic and applied microbiology 2003-11, Vol.26 (4), p.546-556
• Main Authors: Chenoll, E., Macián, M.C., Aznar, R.
• Format: Article
• Language: English
• Subjects: ARDRA; Bacterial Typing Techniques - methods; Bacteriology; Biological and medical sciences; Carnobacterium; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; DNA, Ribosomal - isolation & purification; DNA, Ribosomal Spacer - analysis; DNA, Ribosomal Spacer - isolation & purification; Food Microbiology; food spoilage; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Genetics; Genotype; Gram-Positive Bacteria - classification; Gram-Positive Bacteria - genetics; Gram-Positive Bacteria - isolation & purification; ISR; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Leuconostoc; Leuconostoc - classification; Leuconostoc - genetics; Leuconostoc - isolation & purification; Microbiology; Miscellaneous; Molecular Sequence Data; Pediococcus; Pediococcus - classification; Pediococcus - genetics; Pediococcus - isolation & purification; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; rDNA polymorphism; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Virology
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/072320203770865855

Ribosomal DNA-based techniques including the analysis of profiles generated by ISR amplification, ISR restriction and ARDRA have been evaluated as molecular tools for identifying Carnobacterium, Lactobacillus, Leuconostoc and Pediococcus. They have been applied for the molecular characterization of 91strains with the following identities: eight Carnobacterium including the eight type species of the genus; 61 Lactobacillus including 40 type strains out of 45 species, 13 Leuconostoc, out of them 11 are type strains and three are subspecies of Lc. mesenteroides; and nine strains representing the six species of genus Pediococcus. The genetic relationship displayed between these species by rrn-based profiles is sustained by their phylogenetic relationships and can therefore be considered useful for taxonomic purposes. Profiles obtained by ISR amplification allowed identification at genus level of Carnobacterium and Leuconostoc, and even at species level in genus Carnobacterium. Genera Lactobacillus and Pediococcus could not be distinguished from each other by applying this technique. The Lactobacillus species analysed here (45) were differentiated using ARDRA- Dde I and ISR- Dde I profiles, sequentially, and Pediococcus species by ISR- Dde I profiles. It was necessary to combine profiles generated by restriction of ISR- Dde I, ARDRA- Dde I and ARDRA- Hae III in order to complete the identification of Leuconostoc species.