Characterisation of Persistent and Sporadic Listeria monocytogenes Strains by Pulsed-Field Gel Electrophoresis (PFGE) and Amplified Fragment Length Polymorphism (AFLP)

• Published in: Systematic and applied microbiology 2003-11, Vol.26 (4), p.539-545
• Main Authors: Autio, Tiina, Keto-Timonen, Riikka, Lundén, Janne, Björkroth, Johanna, Korkeala, Hannu
• Format: Article
• Language: English
• Subjects: amplified fragment length polymorphism; Bacterial Typing Techniques - methods; Bacteriology; Biological and medical sciences; DNA Fingerprinting - methods; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Electrophoresis, Gel, Pulsed-Field; Evolution, Molecular; Food Microbiology; Fundamental and applied biological sciences. Psychology; Genetics; Genotype; Listeria monocytogenes; Listeria monocytogenes - classification; Listeria monocytogenes - genetics; Listeria monocytogenes - isolation & purification; Listeria monocytogenes contamination; Microbiology; Miscellaneous; Molecular Epidemiology - methods; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; pulsed-field gel electrophoresis typing; Virology
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1078/072320203770865846

This study was set up to evaluate the genetic similarity or dissimilarity of persistent and sporadic Listeria monocytogenes strains existing in eleven food processing facilities, including fish, dairy, meat and poultry processing plants. In each plant persistent and sporadic strains were selected on the basis of PFGE typing results. A total of 17 strains representing persistent strains and 38 sporadic strains originating from eleven food processing plants were included in the study. PFGE macrorestriction patterns of persistent and sporadic strains from different processing plants were compared and the strains were further studied by amplified fragment length polymorphism (AFLP), being a characterisation method giving more whole genome based information. The 17 persistent and 38 sporadic strains showed 14 and 35 pulsotypes, 14 and 28 AFLP types, respectively. The combination of PFGE and AFLP typing results yielded a total of 48 genotypes. Thirteen of 15 genotypes presented by persistent strains were only associated with persistent strains and similarly 94% (33/35) of genotypes showed by sporadic strains were recovered among sporadic strains only. Our results showed that L. monocytogenes strains causing persistent contamination differ from sporadic strains. In AFLP analysis persistent strains did not, however, form any specific clusters and neither was there any difference between the known two genomic groups. These results indicate that even though persistent strains differ from sporadic strains there seems not to be any specific evolutional lineage of persistent strains.