Anti-Food Allergic Activity of Sulfated Polysaccharide from Gracilaria lemaneiformis is Dependent on Immunosuppression and Inhibition of p38 MAPK

Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column ch...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2016-06, Vol.64 (22), p.4536-4544
Main Authors: Liu, Qing-Mei, Yang, Yang, Maleki, Soheila J, Alcocer, Marcos, Xu, Sha-Sha, Shi, Chao-Lan, Cao, Min-Jie, Liu, Guang-Ming
Format: Article
Language:English
Subjects:
Animals
Anti-Allergic Agents - administration & dosage
Anti-Allergic Agents - chemistry
Female
Food Hypersensitivity - drug therapy
Food Hypersensitivity - genetics
Food Hypersensitivity - immunology
Gracilaria - chemistry
Humans
Immunosuppression
Mast Cells - drug effects
Mast Cells - immunology
Mice
Mice, Inbred BALB C
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - immunology
Plant Extracts - administration & dosage
Plant Extracts - chemistry
Polysaccharides - administration & dosage
Polysaccharides - chemistry
Rats
Seaweed - chemistry
Th2 Cells - drug effects
Th2 Cells - immunology
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Summary:Polysaccharides from Gracilaria lemaneiformis in particular possess various bioactive functions, but their antiallergic activity remains incompletely defined. Sulfated polysaccharide from Gracilaria lemaneiformis (GLSP) was obtained by water extraction and ethanol precipitation followed by column chromatography. BALB/c mice, RBL-2H3, and KU812 cells were used for verifying the anti food allergic activity of GLSP. According to the results of mice experiment, GLSP was able to alleviate allergy symptoms, to reduce TM-specific IgE and IgG1, to suppress Th2 cell polarization, and to promote the function of regulatory T (Treg) cells. In addition, GLSP had the ability to inhibit the function of RBL-2H3 cells. Furthermore, GLSP inhibited the activation of KU812 via suppression of p38 mitogen-activated protein kinase (MAPK). In conclusion, immunosuppression as well as the reduction in the level of p38 MAPK may contribute to GLSP’s putative activity against food allergy. GLSP may be used as a functional food component for allergic patients.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b01086