The effect of cooling management on blood flow to the dominant follicle and estrous cycle length at heat stress

• Published in: Theriogenology 2016-07, Vol.86 (2), p.626-634
• Main Authors: Honig, Hen, Ofer, Lior, Kaim, Moshe, Jacobi, Shamay, Shinder, Dima, Gershon, Eran
• Format: Article
• Language: English
• Subjects: Animal Husbandry; Animals; Cattle - blood; Cattle - physiology; Cold Temperature; Cooling management; Dominant follicle; Doppler index; Estrous cycle; Estrous Cycle - physiology; Female; Hot Temperature; Ovarian Follicle - physiology; Progesterone - blood; Stress, Physiological; Time Factors
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2016.02.017

The use of ultrasound imaging for the examination of reproductive organs has contributed substantially to the fertility management of dairy cows around the world. This method has many advantages such as noninvasiveness and immediate availability of information. Adding Doppler index to the ultrasound imaging examination, improved the estimation of blood volume and flow rate to the ovaries in general and to the dominant follicle in particular. The aim of this study was to examine changes in the blood flow to the dominant follicle and compare them to the follicular development throughout the cycle. We further set out to examine the effects of different types of cooling management during the summer on the changes in blood flow to the dominant follicle. For this purpose, 24 Israeli-Holstein dairy cows, under heat stress, were randomly assigned one of two groups: one was exposed to five cooling sessions per day (5CS) and the other to eight cooling sessions per day (8CS). Blood flow to the dominant follicle was measured daily using Doppler index throughout the estrous cycle. No differences in the preovulatory dominant follicle diameter were detected between the two cooling management regimens during the cycle. However, the length of the first follicular wave was significantly longer, whereas the second follicular wave was nonsignificantly shorter in the 5CS group as compared to the 8CS group. In addition, no difference in blood flow was found during the first 18 days of the cycle between the two groups. However, from Day 20 until ovulation a higher rate of blood flow was measured in the ovaries of cows cooled 8 times per day as compared to the 5CS group. No differences in progesterone levels were noted. Finally, the estrous cycle length was shorter in the 8CS group as compared to the 5CS group. Our data suggest that blood flow to the dominant follicle and estrous cycle length is affected by heat stress. Using the appropriate cooling management during heat stress can enhance the blood flow to the ovary and may contribute to improved fertility in dairy cows.