Simultaneous Quantification of 13 Compounds in Guanxin Shutong Capsule by HPLC Method

• Published in: Journal of chromatographic science 2016-07, Vol.54 (6), p.971-976
• Main Authors: Zhou, Feifei, Zhang, Lunhui, Gu, Liqiang, Zhang, Yuanyuan, Hou, Chenzhi, Bi, Kaishun, Chen, Xiaohui, Zhang, Huifen
• Format: Article
• Language: English
• Subjects: Capsules - chemistry; Chemistry, Pharmaceutical - methods; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal - chemistry
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/bmw029

Guanxin Shutong (GXST) capsule, which is frequently used in clinical therapy, has a certain and positive therapeutic effect against coronary heart disease. However, the existing quality standard of GXST capsule is inadequate to control the quality of GXST capsule. In this paper, a new high-performance liquid chromatographic (HPLC) method for simultaneous determination of 13 compounds (gallic acid, danshensu, protocatechuic acid, procatechuic aldehyde, ellagic acid, rosmarinic acid, salvianolic acid A and salvianolic acid B, eugenol, dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA) in GXST capsule was developed and validated. The analytes were successfully separated and quantified with an Agilent TC-C18 column (250 × 4.6 mm, 5 µm) by gradient elution using 0.05% phosphoric acid and acetonitrile as mobile phase. The flow rate was 1 mL/min and the detection wavelength was set at 280 nm. All the compounds showed good linearity (r > 0.9991) in a relatively wide concentration range. The intra-and the inter-day variability were in the range of 0.85–2.68 and 1.47–2.86%, respectively. The recoveries of the selected compounds were in the range of 95.24–104.75%. This method was successfully applied to quantify the 13 components in GXST capsule and was conducive to controlling the quality of GXST capsule.