Fundamental Connections among Organism C:N:P Stoichiometry, Macromolecular Composition, and Growth

• Published in: Ecology (Durham) 2004-05, Vol.85 (5), p.1217-1229
• Main Authors: Vrede, Tobias, Dobberfuhl, Dean R., S. A. L. M. Kooijman, Elser, James J.
• Format: Article
• Language: English
• Subjects: autotroph production; Autotrophic processes; Autotrophs; Biochemistry; Biomass; Biomass production; C:N:P stoichiometry; Chemical composition; consumer growth rate; dynamic energy budget theory; Ecology; Growth rate; macromolecular allocation patterns; Marine ecology; Nutrients; protein allocation; Proteins; Ribonucleic acid; ribosomal RNA; Ribosomes; RNA; rubisco; Special Feature: Stoichiometric Ecology; Stoichiometry
• ISSN: 0012-9658; 1939-9170
• DOI: 10.1890/02-0249

Whereas it is acknowledged that the C:N:P stoichiometry of consumers and their resources affects both the structure and the function of food webs, and eventually influences broad-scale processes such as global carbon cycles, the mechanistic basis for the variation in stoichiometry has not yet been fully explored. Empirical evidence shows that the specific growth rate is positively related to RNA concentration both between and within taxa in both unicellular and multicellular organisms. Since RNA is rich in P and constitutes a substantial part of the total P in organisms, a high growth rate is also connected with a high P content. We argue that the reason for this pattern is that the growth of all biota is closely linked with their protein synthesis rate, and thus with the concentration of ribosomal RNA. Dynamic energy budget theory supports the positive relationship between RNA and specific growth rate in microorganisms, whereas the predictions concerning multicellulars only partially agrees with the observed pattern. In a simple model of consumer growth, we explore the consequences of various allocation patterns of RNA, protein, carbohydrates/lipids, and other biochemical constituents on organism potential growth rate and C:N:P stoichiometry. According to the model the percentage of N and especially percentage of P per dry mass increases with increasing specific growth rate. Furthermore, the model suggests that macromolecule allocation patterns and thus N:P stoichiometry are allowed to differ substantially at low growth rates whereas the stoichiometry at high growth rates is much more constricted at low N:P. The model fits empirical data reasonably well, but it is also acknowledged that complex life cycles and associated physiological constraints may result in other patterns. We also use a similar approach of modeling organism growth from basic biochemical principles to illustrate fundamental connections among biochemical allocation and C:N stoichiometry in autotroph production, which is based on allocation patterns between carbohydrates and rubisco. Similar to the RNA-protein model, macromolecular composition and C:N ratios are more constrained at high than at low growth rates. The models and the empirical data together suggest that organism growth is tightly linked with the organisms' biochemical and elemental composition. The stoichiometry of growth impinges on nutrient cycles and carbon fluxes at the ecosystem level. Thus, focus on the biological basi