Decreased OxLDL uptake and cholesterol efflux in THP1 cells elicited by cortisol and by cortisone through 11β-hydroxysteroid dehydrogenase type 1

• Published in: Atherosclerosis 2016-07, Vol.250, p.84-94
• Main Authors: Ledda, Angelo, González, Marina, Gulfo, José, Díaz Ludovico, Ivo, Ramella, Nahuel, Toledo, Juan, Garda, Horacio, Grasa, Mar, Esteve, Montserrat
• Format: Article
• Language: English
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase Type 1 - metabolism; 11β-hydroxysteroid dehydrogenase 1; 11β-hydroxysteroid dehydrogenase 2; Cardiovascular; Cholesterol - blood; Cholesterol - metabolism; Cortisol; Cortisone; Cortisone - blood; Cortisone - metabolism; Foam cells; Foam Cells - metabolism; Gene Expression Regulation, Enzymologic; Glucocorticoids - metabolism; Healthy Volunteers; Humans; Hydrocortisone - blood; Hydrocortisone - metabolism; Inflammation; Lipoproteins, LDL - blood; Macrophages - cytology; Macrophages - metabolism; Monocytes - cytology; THP-1 Cells; THP1 macrophages; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0021-9150; 1879-1484
• DOI: 10.1016/j.atherosclerosis.2016.04.020

Abstract Background and aims Data about glucocorticoids role in the development of atherosclerosis are controversial showing different effects in human than in experimental animal models. Atherosclerosis is the result of a chronic inflammatory response to an injured endothelium where an uncontrolled uptake of OxLDL by macrophages triggers the development of foam cells, the main component of fatty streaks in atherosclerotic plaque. There are few data about the direct effect of glucocorticoids in macrophages of atherosclerotic plaque. The aim of the study was to elucidate the role of glucocorticoids in the development of foam cells in atherosclerosis initiation. Methods For this purpose we used THP1 cells differentiated to macrophages with phorbol esters and incubated with OxLDL alone or with cortisol or cortisone. THP1 cells were also incubated with cortisone plus an inhibitor of 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) activity to determine the role of this enzyme on glucocorticoid action in this process. Results Ours results showed that cortisol and cortisone decreased significantly the inflammation promoted by OxLDL, and also diminished the expression of genes involved in influx and efflux of cholesterol resulting in a reduced lipid accumulation. Likewise cortisol and cortisone decreased 11βHSD1 expression in THP1 cells. The presence of the inhibitor of 11βHSD1 abolished all the effects elicited by cortisone. Conclusion Our results indicate a direct effect of glucocorticoids on macrophages braking atherosclerosis initiation, reducing pro-inflammatory markers and OxLDL uptake and cholesterol re-esterification, but also inhibiting cholesterol output. These effects appear to be mediated, at least in part, by 11βHSD1 activity.