High level expression of Streptomyces mobaraensis transglutaminase in Corynebacterium glutamicum using a chimeric pro-region from Streptomyces cinnamoneus transglutaminase

We previously observed secretion of native-type Streptomyces mobaraensis transglutaminase (MTGase) in Corynebacterium glutamicum by co-expressing the subtilisin-like protease SAM-P45 from S. albogriseolus which processes the pro-region. In the present study, we have used a chimeric pro-region consis...

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Bibliographic Details
Published in:Journal of biotechnology 2004-06, Vol.110 (3), p.219-226
Main Authors: Date, Masayo, Yokoyama, Kei-ichi, Umezawa, Yukiko, Matsui, Hiroshi, Kikuchi, Yoshimi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Biotechnology
Biotechnology - methods
Corynebacterium glutamicum
Corynebacterium glutamicum - enzymology
Corynebacterium glutamicum - genetics
Culture Media - analysis
DNA Mutational Analysis
DNA, Bacterial
Fundamental and applied biological sciences. Psychology
Molecular Sequence Data
Mutagenesis, Site-Directed
Plasmids
Protein Sorting Signals
Protein Structure, Tertiary
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - chemistry
Recombinant Fusion Proteins - metabolism
Secretion
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Streptomyces cinnamoneus
Streptomyces mobaraensis
Streptomycetaceae - enzymology
Streptomycetaceae - genetics
Transglutaminase
Transglutaminases - chemistry
Transglutaminases - genetics
Transglutaminases - isolation & purification
Transglutaminases - metabolism
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Summary:We previously observed secretion of native-type Streptomyces mobaraensis transglutaminase (MTGase) in Corynebacterium glutamicum by co-expressing the subtilisin-like protease SAM-P45 from S. albogriseolus which processes the pro-region. In the present study, we have used a chimeric pro-region consisting of S. mobaraensis and Streptomyces cinnamoneus transglutaminases for the production of MTGase in C. glutamicum. As a result, secretion of MTGase using the chimeric pro-region is increased compared to that using the native pro-region.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2004.02.011