indirect method for the rapid estimation of carotenoid contents in Phaeodactylum tricornutum: possible application of other marine algae

The equations of Parsons and Strickland (1963) have been applied to calculate the concn of total carotenoids in P.tricornutum, and the same pigments have simultaneously been determined on the individual fractions after TLC (thin-layer chromatography) separation. Comparison of both sets of data shows...

Full description

Saved in:
Bibliographic Details
Published in:Marine biology 1977-01, Vol.40 (2), p.109-116
Main Authors: Carreto, J.I, Catoggio, J.A
Format: Article
Language:English
Subjects:
Marine
Phaeodactylum tricornutum
plant biochemistry
plant physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The equations of Parsons and Strickland (1963) have been applied to calculate the concn of total carotenoids in P.tricornutum, and the same pigments have simultaneously been determined on the individual fractions after TLC (thin-layer chromatography) separation. Comparison of both sets of data shows that for diverse carotenoids: chlorophyll a ratios, the direct spectrophotometric values range from 80 to 130% of the chromatographic values; wide variations in the proportions of different carotenoids account for such differena. On the basis of correlations between the proportion of each carotenoid and the absorbence ratio 480:663 nm, an indirect method for the rapid estimation of individual carotenoids of P.tricornutum is proposed. Its eventual application to other Chrysophyta spp and to sea water is discussed.
ISSN:0025-3162
1432-1793
DOI:10.1007/BF00396255