Carbon dioxide sequestration by chemolithotrophic oleaginous bacteria for production and optimization of polyhydroxyalkanoate

•Carbon concentrating Serratia sp. ISTD04 was screened for PHA production.•Screening for PHA production was done on the basis of Nile red fluorescence.•GC–MS analysis revealed the presence of 3-hydroxyvalerate, monomer of PHV.•There was a 2 fold increase in PHA production following optimization by R...

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Bibliographic Details
Published in:Bioresource technology 2016-08, Vol.213, p.249-256
Main Authors: Kumar, Manish, Gupta, Asmita, Thakur, Indu Shekhar
Format: Article
Language:English
Subjects:
3-Hydroxyvalerate
Biomass
Biotechnology - methods
Carbon - metabolism
Carbon Dioxide
Carbon Sequestration
CO2 sequestration
Gas Chromatography-Mass Spectrometry
Glucose - metabolism
Oxazines - chemistry
Pentanoic Acids - metabolism
Polyhydroxyalkanoate
Polyhydroxyalkanoates - biosynthesis
Response Surface Methodology
Serratia - metabolism
Serratia sp
Spectroscopy, Fourier Transform Infrared
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Summary:•Carbon concentrating Serratia sp. ISTD04 was screened for PHA production.•Screening for PHA production was done on the basis of Nile red fluorescence.•GC–MS analysis revealed the presence of 3-hydroxyvalerate, monomer of PHV.•There was a 2 fold increase in PHA production following optimization by RSM. The present work involved screening of a previously reported carbon concentrating oleaginous bacterial strain Serratia sp. ISTD04 for production of PHA and optimization of process parameters for enhanced PHA and biomass generation. The selected bacterial strain was screened for PHA production based on Nile red staining followed by visualization under fluorescence microscope. Spectrofluorometric measurement of Nile red fluorescence of the bacterial culture was also done. Confirmatory analysis of PHA accumulation by GC–MS revealed the presence of 3-hydroxyvalerate. Detection of characteristic peaks in the FT-IR spectrum further confirmed the production of PHA by the bacterium. Response Surface Methodology was used for optimization of pH and carbon sources’ concentrations for higher PHA production. There was almost a 2 fold increase in the production of PHA following optimization as compared to un-optimized condition. The study thus establishes the production of PHA by Serratia sp. ISTD04.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2016.02.038