Ozone Therapy in Ethidium Bromide-Induced Demyelination in Rats: Possible Protective Effect

Multiple sclerosis, an autoimmune inflammatory disease of the central nervous system, is characterized by excessive demyelination. The study aimed to investigate the possible protective effect of ozone (O 3 ) therapy in ethidium bromide (EB)-induced demyelination in rats either alone or in combinati...

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Bibliographic Details
Published in:Cellular and molecular neurobiology 2016-08, Vol.36 (6), p.943-954
Main Authors: Salem, Neveen A., Assaf, Naglaa, Ismail, Manal F., Khadrawy, Yasser A., Samy, Mohga
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Demyelinating Diseases - chemically induced
Demyelinating Diseases - drug therapy
Ethidium - toxicity
Interleukin-1beta - metabolism
Male
Motor Activity - drug effects
Motor Activity - physiology
Neurobiology
Neurosciences
Original Research
Oxidants, Photochemical - pharmacology
Ozone - therapeutic use
Rats, Wistar
Tumor Necrosis Factor-alpha - metabolism
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Summary:Multiple sclerosis, an autoimmune inflammatory disease of the central nervous system, is characterized by excessive demyelination. The study aimed to investigate the possible protective effect of ozone (O 3 ) therapy in ethidium bromide (EB)-induced demyelination in rats either alone or in combination with corticosteroids in order to decrease the dose of steroid therapy. Rats were divided into Group (1) normal control rats received saline, Group (2) Sham-operated rats received saline, Group (3) Sham-operated rats received vehicle (oxygen), Group (4) EB-treated rats received EB, Group (5) EB-treated rats received O 3 , Group (6) EB-treated rats received methylprednisolone (MP), and Group (7) EB-treated rats received half the dose of MP concomitant with O 3 . EB-treated rats showed a significant increase in the number of footfalls in the grid walk test, decreased brain GSH, and paraoxonase-1 enzyme activity, whereas brain MDA, TNF-α, IL-1β, INF-γ, Cox-2 immunoreactivity, and p53 protein levels were increased. A significant decline in brain serotonin, dopamine, norepinephrine, and MBP immunoreactivity was also reported. Significant improvement of the above-mentioned parameters was demonstrated with the administration of either MP or O 3 , whereas best amelioration was achieved by combining half the dose of MP with ozone.
ISSN:0272-4340
1573-6830
1573-6830
DOI:10.1007/s10571-015-0279-2