Simultaneous saccharification and fermentation (SSF) of jackfruit seed powder (JFSP) to l-lactic acid and to polylactide polymer

• Published in: Bioresource technology 2016-08, Vol.213, p.283-288
• Main Authors: Nair, Nimisha Rajendran, Nampoothiri, K. Madhavan, Banarjee, Rintu, Reddy, Gopal
• Format: Article
• Language: English
• Subjects: amberlite; Artocarpus - chemistry; Artocarpus - metabolism; Bioplastic; Chromatography, Ion Exchange; Fermentation; Jackfruit seeds; jackfruits; Lactic acid; Lactic Acid - biosynthesis; Lactic Acid - isolation & purification; lactic acid bacteria; liquid state fermentation; Polyesters - metabolism; polylactic acid; Polylactide; polymerization; Powders; Resins, Synthetic - chemistry; saccharification; Seeds - chemistry; Seeds - metabolism; Streptococcus - metabolism; Streptococcus equinus
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2016.03.020

[Display omitted] •Isolation of amylolytic latic acid bacteria.•Simultaneous saccharification and fermentation of jackfruit seed to lactic acid (LA).•Recovery and purification of LA using a week anion exchange resin.•Polymerisation of l-lactic acid to poly (l) lactic acid by polycondensation method. A newly isolated amylolytic lactic acid bacterium, Streptococcus equinus, was used for the production of l-lactic acid from jackfruit seed powder (JFSP) by simultaneous saccharification and fermentation (SSF). After optimization of shake flask fermentation by a response surface box-behnken design, the maximum lactate titer was 109g/L from 200g/L jackfruit seed powder. Amberlite IRA67, a weak base resin, was used to recover pure lactic acid from fermented broth and subsequently used for the synthesis of polylactic acid by direct condensation polymerization method with a yield of 62%.