Clinical significance of ECT2 expression in tissue and serum of gastric cancer patients

The ECT2 (epithelial cell transforming sequence 2) oncogene acted as a guanine nucleotide exchange factor for RhoGTPases, and regulates cytokinesis; thus, it may play a role in the pathogenesis of gastric cancer. In this study, we investigated the expression ECT2 gene in tissues and serum of gastric...

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Bibliographic Details
Published in:Clinical & translational oncology 2016-07, Vol.18 (7), p.735-742
Main Authors: Wang, H.-B., Yan, H.-C., Liu, Y.
Format: Article
Language:English
Subjects:
Adult
Aged
Biomarkers, Tumor - analysis
Blotting, Western
Carcinoma - pathology
Disease Progression
Enzyme-Linked Immunosorbent Assay
Female
Humans
Immunohistochemistry
Male
Medicine
Medicine & Public Health
Middle Aged
Oncology
Proto-Oncogene Proteins - analysis
Proto-Oncogene Proteins - biosynthesis
Research Article
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms - pathology
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Summary:The ECT2 (epithelial cell transforming sequence 2) oncogene acted as a guanine nucleotide exchange factor for RhoGTPases, and regulates cytokinesis; thus, it may play a role in the pathogenesis of gastric cancer. In this study, we investigated the expression ECT2 gene in tissues and serum of gastric cancer patients to explore its clinical significance. ECT2 mRNA expression levels in tissues and serum were examined by RT-PCR, and ECT2 protein expression in tissue was evaluated by Western blot, and was further validated by immunohistochemistry and enzyme-linked immunosorbent assay at serum level. ECT2 level was significantly increased in the GC tissues and serum compared to normal control. ECT2 expression was positively correlated with the histologic differentiation, stages of TNM, and lymph node metastasis in GC ( P  
ISSN:1699-048X
1699-3055
DOI:10.1007/s12094-015-1428-2