Polymorphisms in the long non-coding RNA CDKN2B-AS1 may contribute to higher systolic blood pressure levels in hypertensive patients

• Published in: Clinical biochemistry 2016-07, Vol.49 (10-11), p.821-827
• Main Authors: Bayoglu, Burcu, Yuksel, Husniye, Cakmak, Huseyin Altug, Dirican, Ahmet, Cengiz, Mujgan
• Format: Article
• Language: English
• Subjects: Adult; Aged; Biomarkers - metabolism; Blood Pressure - physiology; Case-Control Studies; CDKN2A/B; CDKN2B-AS1; Chromosome 9p21.3; Female; Follow-Up Studies; Genetic Predisposition to Disease; Genetic variation; Genome-Wide Association Study; Haplotypes; Humans; Hypertension; Hypertension - complications; Hypertension - genetics; Male; Middle Aged; Myocardial Infarction - diagnosis; Myocardial Infarction - etiology; Polymorphism, Single Nucleotide - genetics; Prognosis; Real-Time Polymerase Chain Reaction; Risk Factors; RNA, Long Noncoding - genetics; Turkey
• ISSN: 0009-9120; 1873-2933
• DOI: 10.1016/j.clinbiochem.2016.02.012

Hypertension (HT) is a complex disorder influenced by both genetic and environmental factors. Recent genome-wide association studies have identified a major risk locus for atherosclerosis on chromosome 9p21.3. SNPs within the coding sequences of CDKN2A/B and the long non-coding RNA CDKN2B-AS1 could potentially contribute to HT development. Thus, this study aimed to investigate whether the frequency of four SNPs on chromosome 9p21.3 affects blood pressure (BP) levels in Turkish HT patients, and to examine correlations between these SNPs, specific SNP haplotypes, and HT. This is a case–control study comparing HT patients and healthy controls. Real-time polymerase chain reaction (RT-PCR) analysis was utilized to detect SNPs rs10757274, rs2383207, rs10757278, and rs1333049 in 170 HT patients and 180 healthy controls. Each SNP was detected at significantly higher frequencies in HT patients than in controls (p values 0.001); however, there was no significant link between rs10757274, rs2383207, rs10757278, and rs1333049 SNPs and HT grades. Furthermore, there was a significant association between elevated systolic BP levels and rs1333049 GG genotype (p=0.047), while weight gain and increased fasting glucose levels were significantly associated with rs2383207 AA genotype (p=0.020 and p=0.009, respectively). Lastly, we detected a correlation between GG, GA, and AG haplotypes in block 1 (rs10757274, rs2383207) and GC and AG haplotypes in block 2 (rs10757278, rs1333049) and HT. Our findings suggest that SNPs rs10757274, rs2383207, rs10757278, and rs1333049, particularly those within the CDKN2B-AS1 gene, and related haplotypes may confer increased susceptibility to HT development. •HT is associated with the rs10757274, rs2383207, rs10757278, and rs1333049 SNPs.•The rs1333049 GG genotype correlates with elevated systolic blood pressure levels.•The rs2383207 AA genotype correlates with elevated resting glucose levels.•The GG, GA, and AG haplotypes in block 1 correlate with HT.•The GC and AG haplotypes in block 2 correlate with HT.