Evaluation of four commonly used normalizer genes for the study of decidual gene expression

Abstract Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers ( RNA18S , GAPDH , TBP , and YWHAZ ) across 59 decidual tissue samples col...

Full description

Saved in:
Bibliographic Details
Published in:Placenta (Eastbourne) 2016-07, Vol.43, p.9-12
Main Authors: Sequeira de Sousa, Ana Rita, Staff, Anne Cathrine, Johnsen, Guro Mørk, Weedon-Fekjær, Mina Susanne, Størvold, Gro Leite
Format: Article
Language:English
Subjects:
14-3-3 Proteins - genetics
14-3-3 Proteins - metabolism
Decidua - metabolism
Decidua basalis
Female
Gene Expression
Gene Expression Profiling - methods
Genes, Essential - genetics
Housekeeping gene
Humans
Internal Medicine
Normalizer gene
Obstetrics and Gynecology
Preeclampsia
Quantitative PCR
Real-Time Polymerase Chain Reaction - methods
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Ribosomal, 18S - genetics
RNA, Ribosomal, 18S - metabolism
TATA-Box Binding Protein - genetics
TATA-Box Binding Protein - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Reverse transcription quantitative PCR (RT-qPCR) gene expression results must be normalized using stably expressed genes to correct for technical variation. We evaluated the expression of four widely used normalizers ( RNA18S , GAPDH , TBP , and YWHAZ ) across 59 decidual tissue samples collected by vacuum suction from preeclamptic and normotensive pregnancies. RNA18S and GAPDH were not suitable as normalizers, while YWHAZ and TBP were stably expressed across the study groups.
ISSN:0143-4004
1532-3102
DOI:10.1016/j.placenta.2016.04.009