Structure and mode of transposition of Tn2555 carrying sucrose utilization genes

The sucrose transposon Tn2555 from Escherichia coli, which has an unstable structure, was studied in more detail. Sequence analysis of one of the transposon variants, designated Tn2555.3, revealed the presence of two direct IS26 copies on its flanks, and a third inverted IS26 copy inside the transpo...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-04, Vol.233 (2), p.353-359
Main Authors: Doroshenko, Vera G, Livshits, Vitaliy A
Format: Article
Language:English
Subjects:
Escherichia coli
IS26
Sucrose utilization
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Summary:The sucrose transposon Tn2555 from Escherichia coli, which has an unstable structure, was studied in more detail. Sequence analysis of one of the transposon variants, designated Tn2555.3, revealed the presence of two direct IS26 copies on its flanks, and a third inverted IS26 copy inside the transposon. The sucrose utilization genes of Tn2555.3 were found to be identical to those of the previously described pUR400 plasmid. It was demonstrated that Tn2555.3 translocation from pBR325 to RP4 occurs via a cointegrate formation, mediated by one of the three IS26 copies, followed by its resolution due to RecA-dependent recombination between two direct IS26 copies flanking the donor replicon.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.03.016