Validated UPLC-MS/MS Methods To Quantitate Free and Conjugated Alternaria Toxins in Commercially Available Tomato Products and Fruit and Vegetable Juices in Belgium

• Published in: Journal of agricultural and food chemistry 2016-06, Vol.64 (24), p.5101-5109
• Main Authors: Walravens, Jeroen, Mikula, Hannes, Rychlik, Michael, Asam, Stefan, Devos, Tom, Njumbe Ediage, Emmanuel, Diana Di Mavungu, José, Jacxsens, Liesbeth, Van Landschoot, Anita, Vanhaecke, Lynn, De Saeger, Sarah
• Format: Article
• Language: English
• Subjects: Alternaria - chemistry; Alternaria - metabolism; Belgium; Chromatography, High Pressure Liquid - methods; Food Contamination - analysis; Food Contamination - economics; Fruit and Vegetable Juices - analysis; Fruit and Vegetable Juices - economics; Fruit and Vegetable Juices - microbiology; Mycotoxins - chemistry; Tandem Mass Spectrometry - methods
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.6b01029

Ultraperformance liquid chromatography tandem mass spectrometry and Quick, Easy, Cheap, Effective, Rugged, and Safe based analytical methodologies to quantitate both free (alternariol (1), alternariol monomethyl ether (2), tenuazonic acid (3), tentoxin (4), altenuene (5), altertoxin-I (6)) and conjugated (sulfates and glucosides of 1 and 2) Alternaria toxins in fruit and vegetable juices and tomato products were developed and validated. Acceptable limits of quantitation (0.7–5.7 μg/kg), repeatability (RSDr < 15.7%), reproducibility (RSDR < 17.9%), and apparent recovery (87.0–110.6%) were obtained for all analytes in all matrices investigated. 129 commercial foodstuffs were analyzed, and 3 was detected in 100% of tomato product samples (