Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A

This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by c...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-04, Vol.233 (2), p.233-240
Main Authors: Huang, I-Hsiu, Waters, Michael, Grau, Roberto R, Sarker, Mahfuzur R
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacterial Proteins - genetics
Base Sequence
Biological and medical sciences
C. perfringens
Clostridium perfringens
Clostridium perfringens - genetics
Clostridium perfringens - growth & development
Clostridium perfringens - metabolism
Enterotoxin
Enterotoxins - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Microbiology
Molecular Sequence Data
Mutagenesis
Plasmids
spo0A
Spores, Bacterial - genetics
Sporulation
Transcription Factors - genetics
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Summary:This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type spo0A gene. These results provide evidence that spo0A expression is essential for sporulation and enterotoxin production in C. perfringens.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.02.014