Loading…
Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A
This study identified a functional spo0A ORF in enterotoxigenic Clostridium perfringens type A. To evaluate the function of spo0A, an isogenic spo0A knock-out mutant was constructed. The spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by c...
Saved in:
| Published in: | FEMS microbiology letters 2004-04, Vol.233 (2), p.233-240 |
|---|---|
| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | 240 |
| container_issue | 2 |
| container_start_page | 233 |
| container_title | FEMS microbiology letters |
| container_volume | 233 |
| creator | Huang, I-Hsiu Waters, Michael Grau, Roberto R Sarker, Mahfuzur R |
| description | This study identified a functional
spo0A ORF in enterotoxigenic
Clostridium perfringens type A. To evaluate the function of
spo0A, an isogenic
spo0A knock-out mutant was constructed. The
spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type
spo0A gene. These results provide evidence that
spo0A expression is essential for sporulation and enterotoxin production in
C. perfringens. |
| doi_str_mv | 10.1016/j.femsle.2004.02.014 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_18002775</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378109704001569</els_id><sourcerecordid>18002775</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-a3b631ad0c317d8b618d3969dccfb5f0434c2c185ef2ab7e998257008c6a6c593</originalsourceid><addsrcrecordid>eNp9kc-O1DAMxiMEYoeFN0AoFxAcWpw2TdvLSqPhr7QSFzhHaeIsGdqmJCliH4s3JLMdaTlxsmz_PtvyR8hzBiUDJt4eS4tTHLGsAHgJVQmMPyA71rS8EL3oHpId1G1XMOjbC_IkxiNksALxmFywBkTNe7Yjf965GNYlOT9Tb2n6jvQGZ6SvaVw87N9QnLU3br455WEd1R2ZgpqjDm7TWaWTD3QYvf4Rs8D4E4vU-jBtvJpNricMPvnfbqZL8GbVd62c3XfyaqfpYfQxBWfcOtEFgw15Pc6RptsF6f4peWTVGPHZOV6Sbx_efz18Kq6_fPx82F8XmjOeClUPombKgK5Za7pBsM7UveiN1nZoLPCa60qzrkFbqaHFvu-qpgXotFBCN319SV5tc_OxP1eMSU4uahxHNaNfo2QdQNW2TQb5BurgYwxo5RLcpMKtZCBPVsmj3KySJ6skVDJblWUvzvPXYUJzLzp7k4GXZ0BFrUabf65d_IcTdcfrNnNXG4f5G78cBhm1y7ahcQF1ksa7_1_yF7douTU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18002775</pqid></control><display><type>article</type><title>Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A</title><source>Oxford Journals Online</source><creator>Huang, I-Hsiu ; Waters, Michael ; Grau, Roberto R ; Sarker, Mahfuzur R</creator><creatorcontrib>Huang, I-Hsiu ; Waters, Michael ; Grau, Roberto R ; Sarker, Mahfuzur R</creatorcontrib><description>This study identified a functional
spo0A ORF in enterotoxigenic
Clostridium perfringens type A. To evaluate the function of
spo0A, an isogenic
spo0A knock-out mutant was constructed. The
spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type
spo0A gene. These results provide evidence that
spo0A expression is essential for sporulation and enterotoxin production in
C. perfringens.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2004.02.014</identifier><identifier>PMID: 15063491</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Bacterial Proteins - genetics ; Base Sequence ; Biological and medical sciences ; C. perfringens ; Clostridium perfringens ; Clostridium perfringens - genetics ; Clostridium perfringens - growth & development ; Clostridium perfringens - metabolism ; Enterotoxin ; Enterotoxins - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Microbiology ; Molecular Sequence Data ; Mutagenesis ; Plasmids ; spo0A ; Spores, Bacterial - genetics ; Sporulation ; Transcription Factors - genetics</subject><ispartof>FEMS microbiology letters, 2004-04, Vol.233 (2), p.233-240</ispartof><rights>2004 Federation of European Microbiological Societies</rights><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15638437$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15063491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, I-Hsiu</creatorcontrib><creatorcontrib>Waters, Michael</creatorcontrib><creatorcontrib>Grau, Roberto R</creatorcontrib><creatorcontrib>Sarker, Mahfuzur R</creatorcontrib><title>Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>This study identified a functional
spo0A ORF in enterotoxigenic
Clostridium perfringens type A. To evaluate the function of
spo0A, an isogenic
spo0A knock-out mutant was constructed. The
spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type
spo0A gene. These results provide evidence that
spo0A expression is essential for sporulation and enterotoxin production in
C. perfringens.</description><subject>Amino Acid Sequence</subject><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>C. perfringens</subject><subject>Clostridium perfringens</subject><subject>Clostridium perfringens - genetics</subject><subject>Clostridium perfringens - growth & development</subject><subject>Clostridium perfringens - metabolism</subject><subject>Enterotoxin</subject><subject>Enterotoxins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis</subject><subject>Plasmids</subject><subject>spo0A</subject><subject>Spores, Bacterial - genetics</subject><subject>Sporulation</subject><subject>Transcription Factors - genetics</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kc-O1DAMxiMEYoeFN0AoFxAcWpw2TdvLSqPhr7QSFzhHaeIsGdqmJCliH4s3JLMdaTlxsmz_PtvyR8hzBiUDJt4eS4tTHLGsAHgJVQmMPyA71rS8EL3oHpId1G1XMOjbC_IkxiNksALxmFywBkTNe7Yjf965GNYlOT9Tb2n6jvQGZ6SvaVw87N9QnLU3br455WEd1R2ZgpqjDm7TWaWTD3QYvf4Rs8D4E4vU-jBtvJpNricMPvnfbqZL8GbVd62c3XfyaqfpYfQxBWfcOtEFgw15Pc6RptsF6f4peWTVGPHZOV6Sbx_efz18Kq6_fPx82F8XmjOeClUPombKgK5Za7pBsM7UveiN1nZoLPCa60qzrkFbqaHFvu-qpgXotFBCN319SV5tc_OxP1eMSU4uahxHNaNfo2QdQNW2TQb5BurgYwxo5RLcpMKtZCBPVsmj3KySJ6skVDJblWUvzvPXYUJzLzp7k4GXZ0BFrUabf65d_IcTdcfrNnNXG4f5G78cBhm1y7ahcQF1ksa7_1_yF7douTU</recordid><startdate>20040415</startdate><enddate>20040415</enddate><creator>Huang, I-Hsiu</creator><creator>Waters, Michael</creator><creator>Grau, Roberto R</creator><creator>Sarker, Mahfuzur R</creator><general>Elsevier B.V</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20040415</creationdate><title>Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A</title><author>Huang, I-Hsiu ; Waters, Michael ; Grau, Roberto R ; Sarker, Mahfuzur R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-a3b631ad0c317d8b618d3969dccfb5f0434c2c185ef2ab7e998257008c6a6c593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>C. perfringens</topic><topic>Clostridium perfringens</topic><topic>Clostridium perfringens - genetics</topic><topic>Clostridium perfringens - growth & development</topic><topic>Clostridium perfringens - metabolism</topic><topic>Enterotoxin</topic><topic>Enterotoxins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis</topic><topic>Plasmids</topic><topic>spo0A</topic><topic>Spores, Bacterial - genetics</topic><topic>Sporulation</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, I-Hsiu</creatorcontrib><creatorcontrib>Waters, Michael</creatorcontrib><creatorcontrib>Grau, Roberto R</creatorcontrib><creatorcontrib>Sarker, Mahfuzur R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, I-Hsiu</au><au>Waters, Michael</au><au>Grau, Roberto R</au><au>Sarker, Mahfuzur R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2004-04-15</date><risdate>2004</risdate><volume>233</volume><issue>2</issue><spage>233</spage><epage>240</epage><pages>233-240</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>This study identified a functional
spo0A ORF in enterotoxigenic
Clostridium perfringens type A. To evaluate the function of
spo0A, an isogenic
spo0A knock-out mutant was constructed. The
spo0A mutant was unable to form endospores and produce enterotoxin, however, these defects could be restored by complementing the mutant with a recombinant plasmid carrying the wild-type
spo0A gene. These results provide evidence that
spo0A expression is essential for sporulation and enterotoxin production in
C. perfringens.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>15063491</pmid><doi>10.1016/j.femsle.2004.02.014</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2004-04, Vol.233 (2), p.233-240 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_18002775 |
| source | Oxford Journals Online |
| subjects | Amino Acid Sequence Bacterial Proteins - genetics Base Sequence Biological and medical sciences C. perfringens Clostridium perfringens Clostridium perfringens - genetics Clostridium perfringens - growth & development Clostridium perfringens - metabolism Enterotoxin Enterotoxins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Microbiology Molecular Sequence Data Mutagenesis Plasmids spo0A Spores, Bacterial - genetics Sporulation Transcription Factors - genetics |
| title | Disruption of the gene ( spo0A) encoding sporulation transcription factor blocks endospore formation and enterotoxin production in enterotoxigenic Clostridium perfringens type A |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T00%3A27%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Disruption%20of%20the%20gene%20(%20spo0A)%20encoding%20sporulation%20transcription%20factor%20blocks%20endospore%20formation%20and%20enterotoxin%20production%20in%20enterotoxigenic%20Clostridium%20perfringens%20type%20A&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Huang,%20I-Hsiu&rft.date=2004-04-15&rft.volume=233&rft.issue=2&rft.spage=233&rft.epage=240&rft.pages=233-240&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1016/j.femsle.2004.02.014&rft_dat=%3Cproquest_cross%3E18002775%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c414t-a3b631ad0c317d8b618d3969dccfb5f0434c2c185ef2ab7e998257008c6a6c593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=18002775&rft_id=info:pmid/15063491&rfr_iscdi=true |