SUBSTITUTION REACTIONS IN APCI NEGATIVE MASS SPECTROMETRY: ANALYSIS OF 1-CHLORO-2-NITROBENZENE IN QUETIAPINE FUMARATE

A new, sensitive, and rapid UPLC-MS method for the determination of 1-chloro-2-nitrobenzene in ultra performance liquid chromatography mass spectrometry using substitution reactions in atmospheric pressure chemical ionization negative mode [APCI (-)] is described. Substitution reactions [M - X + O]...

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Bibliographic Details
Published in:Journal of liquid chromatography & related technologies 2014-09, Vol.37 (15), p.2131-2141
Main Authors: Gudlawar, Shiva Kumar, Dwivedi, Jaya
Format: Article
Language:English
Subjects:
1-chloro-2-nitrobenzene
APCI negative mode
Atmospheric pressure
Barometric pressure
Chromatography
Ionization
Liquid chromatography
Low level
Mass spectrometry
Mathematical analysis
single reaction monitoring (SIR)
Substitution reactions
TTC
UPLC-MS
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Summary:A new, sensitive, and rapid UPLC-MS method for the determination of 1-chloro-2-nitrobenzene in ultra performance liquid chromatography mass spectrometry using substitution reactions in atmospheric pressure chemical ionization negative mode [APCI (-)] is described. Substitution reactions [M - X + O] − are observed for compounds which do not easily undergo deprotonation. By using threshold of toxicological concern (TTC), a limit of 1.63 ppm was calculated based on maximum daily dose of the drug substance. The proposed method was found to be linear from 50.0 to 2000.0 ppb with correlation coefficient >0.99. LOD and LOQ of the method were very low as 20.0 and 50.0 ppb. The accuracy of the method was found to be in the range of 85-105%. This method is a good quality control tool for quantification of 1-chloro-2-nitrobenzene at low levels in quetiapine fumarate.
ISSN:1082-6076
1520-572X
DOI:10.1080/10826076.2013.825867