DNA Cleavage Activity: Comparison of two Lanthanum Complexes Based on Aza-Crown Ethers with Different Numbers of Nitrogen Atoms

Based on the unique characteristics of lanthanum ion and aza-crown ethers, the lanthanum complexes of two aza-crown ether (L1: 1,10-Dioxa-4,7,13,16-tetraazacyclo-octadecane and L2: 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane) were designed and synthesised. The interaction between these two complexe...

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Bibliographic Details
Published in:Progress in reaction kinetics and mechanism 2015-01, Vol.40 (4), p.391-401
Main Authors: Yu, Lan, Li, Fang-zhen, Xie, Jia-qing, Cai, Shulan
Format: Article
Language:English
Subjects:
Catalysis
Catalysts
Cleavage
Deoxyribonucleic acid
Ethers
Lanthanum
Nitrogen atoms
Optimization
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Summary:Based on the unique characteristics of lanthanum ion and aza-crown ethers, the lanthanum complexes of two aza-crown ether (L1: 1,10-Dioxa-4,7,13,16-tetraazacyclo-octadecane and L2: 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane) were designed and synthesised. The interaction between these two complexes and DNA was measured by UV-Vis spectroscopy and gel electrophoresis. Moreover, a series of experiments of cleavage of pUC19 DNA were conducted to illustrate the acidity, time and concentration effects. The results indicated that the two metal complexes can accelerate the breakage of DNA from its supercoiled form (form I) to the nicked form (form II) at near-physiological conditions, and the optimum acidity of DNA catalytic cleavage is pH=6.5 and pH=7.0 for LaL1 and LaL2, respectively. Furthermore, the LaL1 exhibited better cleavage activity than LaL2 under the same conditions, thus supercoiled DNA was thoroughly cleaved to the nicked form under the appropriate conditions. The hydrolytic mechanism was verified by applying several oxygen-scavengers to the DNA catalytic cleavage.
ISSN:1468-6783
1471-406X
DOI:10.3184/146867815X14420468512656