Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highl...

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Bibliographic Details
Published in:Analytical Sciences 2015/11/10, Vol.31(11), pp.1189-1192
Main Authors: MURAKAMI, Hiroya, OTANI, Erina, IWATA, Tomoko, ESAKA, Yukihiro, AOYAMA, Takuma, KAWASAKI, Masanori, TANAKA, Toshiki, MINATOGUCHI, Shinya, UNO, Bunji
Format: Article
Language:English
Subjects:
Adenosine
Adenosine - analysis
Adenosine - isolation & purification
Adenosines
ammonium acetate
Analytic Sample Preparation Methods - methods
Analytical Chemistry
Blood Chemical Analysis - methods
Calibration
Chemistry
Chromatography
Chromatography, Liquid - methods
HILIC separation
Human
Human behavior
human plasma
Humans
Hydrophobic and Hydrophilic Interactions
ion suppression
LC-ESI-MS/MS
Mathematical analysis
Medical services
Pretreatment
Separation
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry
Ultrafiltration
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Summary:A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.31.1189