Polylysine-modified Polyethylenimines as siRNA Carriers for Effective Tumor Treatment

Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and conf...

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Bibliographic Details
Published in:Chinese journal of polymer science 2015-06, Vol.33 (6), p.830-837
Main Authors: Chen, Jie, Jiao, Zi-xue, Lin, Lin, Guo, Zhao-pei, Xu, Cai-na, Li, Yan-hui, Tian, Hua-yu, Chen, Xue-si
Format: Article
Language:English
Subjects:
Carriers
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Complexation
Condensed Matter Physics
Confocal
Copolymers
Genes
Industrial Chemistry/Chemical Engineering
L-赖氨酸
Particle size
Polymer Sciences
siRNA
Tumors
Zeta potential
zeta电位
流式细胞分析
聚乙烯亚胺
肿瘤治疗
载体
酸改性
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Summary:Polyethylenimine-poly（L-lysine） （PEI-PLL） copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride （Lys（Z）-NCA） initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.
ISSN:0256-7679
1439-6203
DOI:10.1007/s10118-015-1632-0