Capturing in Vivo Plant Metabolism by Real-Time Analysis of Low to High Molecular Weight Volatiles

We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day–night cycles. To...

Full description

Saved in:
Bibliographic Details
Published in:Analytical chemistry (Washington) 2016-02, Vol.88 (4), p.2406-2412
Main Authors: Barrios-Collado, César, García-Gómez, Diego, Zenobi, Renato, Vidal-de-Miguel, Guillermo, Ibáñez, Alfredo J, Martinez-Lozano Sinues, Pablo
Format: Article
Language:English
Subjects:
Analytical chemistry
Analyzers
Begonia
Begoniaceae - chemistry
Begoniaceae - metabolism
Chromatography
Complement
Emission analysis
Emissions
In vivo methods and tests
Ionization
Light
Mass spectrometry
Molecular Weight
Photosynthesis
Spectrometry, Mass, Electrospray Ionization - instrumentation
Time Factors
VOCs
Volatile organic compounds
Volatile Organic Compounds - analysis
Volatile Organic Compounds - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have deployed an efficient secondary electrospray ionization source coupled to an Orbitrap mass analyzer (SESI-MS) to investigate the emissions of a Begonia semperflorens. We document how hundreds of species can be tracked with an unparalleled time resolution of 2 min during day–night cycles. To further illustrate the capabilities of this system for volatile organic compounds (VOCs) analysis, we subjected the plant to mechanical damage and monitored its response. As a result, ∼1200 VOCs were monitored displaying different kinetics. To validate the soundness of our in vivo measurements, we fully characterized some key compounds via tandem mass spectrometry (MS/MS) and confirmed their expected behavior based on prior gas chromatography/mass spectrometry (GC/MS) studies. For example, β-caryophyllene, which is directly related to photosynthesis, was found to show a periodic day–night pattern with highest concentrations during the day. We conclude that the capability of SESI-MS to capture highly dynamic VOC emissions and wide analyte coverage makes it an attractive tool to complement GC/MS in plant studies.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.5b04452