A portable electrochemical immunosensor for rapid detection of trace aflatoxin B sub(1) in rice

To explore the possibility of achieving a rapid and in situ detection of aflatoxin B sub(1) (AFB sub(1)), a portable biosensing instrument consisting of an impedance detector and a 3D-printed USB-compatible sensor chip was developed. In this study, we proposed an electrochemical biosensor based on s...

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Bibliographic Details
Published in:Analytical methods 2016-01, Vol.8 (3), p.548-553
Main Authors: Li, Zhanming, Ye, Zunzhong, Fu, Yingchun, Xiong, Yonghua, Li, Yanbin
Format: Article
Language:English
Subjects:
Aflatoxins
Biosensors
Detectors
Immunosensors
Mathematical analysis
Microelectrodes
Portability
Stations
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Summary:To explore the possibility of achieving a rapid and in situ detection of aflatoxin B sub(1) (AFB sub(1)), a portable biosensing instrument consisting of an impedance detector and a 3D-printed USB-compatible sensor chip was developed. In this study, we proposed an electrochemical biosensor based on screen-printed interdigitated microelectrodes (SPIMs) and a portable detector to achieve low-cost, highly selective and sensitive detection of AFB sub(1) in rice. Under optimized conditions, the immunosensor provided a detection limit of 5 ng mL super(-1), which was below the allowable concentration. The total detection time including incubation was less than 1 h. The results obtained from this developed portable detection system were comparable to those from a commercial electrochemical station in the laboratory. Hence, the portable detector offers new tools for detection of a wide variety of analytes in clinical and environmental samples.
ISSN:1759-9660
1759-9679
DOI:10.1039/c5ay02643a