Tilted pillar array fabrication by the combination of proton beam writing and soft lithography for microfluidic cell capture: Part 1 Design and feasibility

Design, fabrication, integration, and feasibility test results of a novel microfluidic cell capture device is presented, exploiting the advantages of proton beam writing to make lithographic irradiations under multiple target tilting angles and UV lithography to easily reproduce large area structure...

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Bibliographic Details
Published in:Electrophoresis 2016-02, Vol.37 (3), p.498-503
Main Authors: Rajta, Istvan, Huszánk, Robert, Szabó, Atilla T. T, Nagy, Gyula U. L, Szilasi, Szabolcs, Fürjes, Peter, Holczer, Eszter, Fekete, Zoltan, Járvás, Gabor, Szigeti, Marton, Hajba, Laszlo, Bodnár, Judit, Guttman, Andras
Format: Article
Language:English
Subjects:
Arrays
Cell capture
cell culture
Cell Separation - instrumentation
Cell Separation - methods
coatings
Design engineering
Devices
electrophoresis
Equipment Design
erythrocytes
Feasibility Studies
Fluid dynamics
Fluid flow
hydrodynamics
Lithography
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Microfluidics
Models, Biological
Proton beam writing
Proton beams
Soft lithography
yeasts
Yeasts - cytology
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Summary:Design, fabrication, integration, and feasibility test results of a novel microfluidic cell capture device is presented, exploiting the advantages of proton beam writing to make lithographic irradiations under multiple target tilting angles and UV lithography to easily reproduce large area structures. A cell capture device is demonstrated with a unique doubly tilted micropillar array design for cell manipulation in microfluidic applications. Tilting the pillars increased their functional surface, therefore, enhanced fluidic interaction when special bioaffinity coating was used, and improved fluid dynamic behavior regarding cell culture injection. The proposed microstructures were capable to support adequate distribution of body fluids, such as blood, spinal fluid, etc., between the inlet and outlet of the microfluidic sample reservoirs, offering advanced cell capture capability on the functionalized surfaces. The hydrodynamic characteristics of the microfluidic systems were tested with yeast cells (similar size as red blood cells) for efficient capture.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201500254