Depletion of enteroendocrine and mucus-secreting cells is associated with colorectal carcinogenesis severity and impaired intestinal motility in rats

• Published in: Microscopy research and technique 2016-01, Vol.79 (1), p.3-13
• Main Authors: Novaes, Rômulo Dias, Sequetto, Priscila Lima, Vilela Gonçalves, Reggiani, Cupertino, Marli Carmo, Santos, Eliziária Cardoso, Mello, Vanessa Joia, Araújo, Marta Rocha, Silva, Edson, Oliveira, Tânia Toledo
• Format: Article
• Language: English
• Subjects: 1,2-Dimethylhydrazine - toxicity; aberrant crypt foci; Aberrant Crypt Foci - pathology; Animals; Carcinogens; Colorectal Neoplasms - chemically induced; Colorectal Neoplasms - pathology; Correlation; Depletion; Disease Models, Animal; Enteric Nervous System; Enteroendocrine Cells - pathology; Evolutionary; Female; Gastrointestinal Motility - physiology; histomorphometry; intestinal epithelium; Inverse; large intestine; Rats; Rats, Wistar; Receiving
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.22534

ABSTRACT This study investigated the relationship between enteroendocrine and mucus‐secreting cells distribution, the severity of colonic mucosal injury and intestinal motility in experimental colorectal carcinogenesis. Using a standardized murine model of colorectal carcinogenesis, eight‐weeks‐old female Wistar rats weighting 147.30 ± 29.15g were randomized into two groups receiving a subcutaneous injection of 0.9% saline (control) or the chemical carcinogen 1,2‐dimethylhydrazine (DMH) at 20 mg/kg per week during 10 weeks. Aberrant crypt foci (ACF) were more frequent in DMH group compared to control group (P