Photoinduced and Self-Activated Nuclease Activity of Copper(II) Complexes with N-(Quinolin-8-yl)quin­olin-8-sulfonamide - DNA and Bovine Serum Albumin Binding

Two CuII complexes with a new quinoline sulfonamide derivative and phenanthroline (phen), [Cu(QSQ)(phen)]ClO4·0.5H2O (1) and [Cu(QSQ)(phen)(H2O)]ClO4 (2) [HQSQ = N‐(quinolin‐8‐yl)quinolin‐8‐sulfonamide], have been synthesized and physicochemically characterized. Single‐crystal X‐ray diffraction stud...

Full description

Saved in:
Bibliographic Details
Published in:European journal of inorganic chemistry 2016-03, Vol.2016 (7), p.982-994
Main Authors: Pascual-Álvarez, Alejandro, Topala, Tamara, Estevan, Francisco, Sanz, Francisca, Alzuet-Piña, Gloria
Format: Article
Language:English
Subjects:
Antitumor agents
Binding
Cleavage
Copper
Deoxyribonucleic acid
DNA
DNA binding
DNA cleavage
Fluorescence
Medicinal chemistry
Nuclease
Protein binding
Proteins
Reagents
Serum albumin
Viscometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Two CuII complexes with a new quinoline sulfonamide derivative and phenanthroline (phen), [Cu(QSQ)(phen)]ClO4·0.5H2O (1) and [Cu(QSQ)(phen)(H2O)]ClO4 (2) [HQSQ = N‐(quinolin‐8‐yl)quinolin‐8‐sulfonamide], have been synthesized and physicochemically characterized. Single‐crystal X‐ray diffraction studies have revealed a highly distorted trigonal‐bipyramidal structure for 1 (τ = 0.68) and an almost perfect trigonal‐bipyramidal geometry for 2 (τ = 0.92). DNA binding studies, which were performed by thermal denaturation, viscometry, fluorescence spectroscopy, and cyclic voltammetry, indicated a partial intercalation of 1 with Kapp = 2.45 × 106 M–1. The nuclease activity of 1 was investigated upon photoirradiation, with ascorbate/H2O2 as the activating agent, and also in the absence of any external reagent. In all cases, 1 was able to perform DNA cleavage, and its nuclease efficiency varied in the order ascorbate/H2O2 > photoirradiation > without external cofactors. Mechanistic investigations suggest an oxidative cleavage of DNA involving reactive oxygen species (ROS). The protein binding ability of 1 was also studied with bovine serum albumin (BSA) as a model protein. [Cu(QSQ)(phen)]ClO4·0.5H2O (1) and [Cu(QSQ)(phen)(H2O)]ClO4 [2; phen = phenanthroline, HQSQ = N‐(quinolin‐8‐yl)quinolin‐8‐sulfonamide] are synthesized and physicochemically characterized. Compound 1 performs oxidative DNA cleavage upon photoirradiation in the presence of ascorbate/H2O2 and, interestingly, in the absence of any external reagent.
ISSN:1434-1948
1099-0682
DOI:10.1002/ejic.201501469