Stainless steel surface functionalization for immobilization of antibody fragments for cardiovascular applications

• Published in: Journal of biomedical materials research. Part A 2016-04, Vol.104 (4), p.821-832
• Main Authors: Foerster, A., Hołowacz, I., Sunil Kumar, G. B., Anandakumar, S., Wall, J. G., Wawrzyńska, M., Paprocka, M., Kantor, A., Kraskiewicz, H., Olsztyńska-Janus, S., Hinder, S. J., Bialy, D., Podbielska, H., Kopaczyńska, M.
• Format: Article
• Language: English
• Subjects: aminosilanization; Antibodies, Immobilized - chemistry; antibody fragments; Austenitic stainless steels; Cell Adhesion; Cell Line; Cell Proliferation; Coated Materials, Biocompatible - chemistry; Coating; Density; Endothelial Progenitor Cells - cytology; Fragments; Humans; immobilization; Materials Testing; progenitor cells; Propylamines - chemistry; Recombinant Proteins - chemistry; Silanes - chemistry; Single-Chain Antibodies - chemistry; Stainless Steel - chemistry; Stainless steels; Stents; surface modification; Surface Properties; Surgical implants; Titanium dioxide
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35616

Stainless steel 316 L material is commonly used for the production of coronary and peripheral vessel stents. Effective biofunctionalization is a key to improving the performance and safety of the stents after implantation. This paper reports the method for the immobilization of recombinant antibody fragments (scFv) on stainless steel 316 L to facilitate human endothelial progenitor cell (EPC) growth and thus improve cell viability of the implanted stents for cardiovascular applications. The modification of stent surface was conducted in three steps. First the stent surface was coated with titania based coating to increase the density of hydroxyl groups for successful silanization. Then silanization with 3 aminopropyltriethoxysilane (APTS) was performed to provide the surface with amine groups which presence was verified using FTIR, XPS, and fluorescence microscopy. The maximum density of amine groups (4.8*10−5 mol/cm2) on the surface was reached after reaction taking place in ethanol for 1 h at 60° C and 0.04M APTS. On such prepared surface the glycosylated scFv were subsequently successfully immobilized. The influence of oxidation of scFv glycan moieties and the temperature on scFv coating were investigated. The fluorescence and confocal microscopy study indicated that the densest and most uniformly coated surface with scFv was obtained at 37°C after oxidation of glycan chain. The results demonstrate that the scFv cannot be efficiently immobilized without prior aminosilanization of the surface. The effect of the chemical modification on the cell viability of EPC line 55.1 (HucPEC‐55.1) was performed indicating that the modifications to the 316 L stainless steel are non‐toxic to EPCs. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 821–832, 2016.