Construction of Insulin 18-mer Nanoassemblies Driven by Coordination to Iron(II) and Zinc(II) Ions at Distinct Sites

Controlled self‐assembly (SA) of proteins offers the possibility to tune their properties or to create new materials. Herein, we present the synthesis of a modified human insulin (HI) with two distinct metal‐ion binding sites, one native, the other abiotic, enabling hierarchical SA through coordinat...

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Bibliographic Details
Published in:Angewandte Chemie 2016-02, Vol.128 (7), p.2424-2427
Main Authors: Munch, Henrik K., Nygaard, Jesper, Christensen, Niels Johan, Engelbrekt, Christian, Østergaard, Mads, Porsgaard, Trine, Hoeg-Jensen, Thomas, Zhang, Jingdong, Arleth, Lise, Thulstrup, Peter W., Jensen, Knud J.
Format: Article
Language:eng ; ger
Subjects:
Affinity
Attachment
Binding sites
Chemical synthesis
Chemistry
Construction
Formations
Hexamers
Insulin
Ions
Iron
Kleinwinkel-Röntgenstreuung
Ligands
Materials selection
Metal ions
Metals
Nanostructure
Nanostrukturen
Nanotechnology
Oligomers
Proteins
Rastersondenverfahren
Scattering
Selbstorganisation
Self assembly
Small angle X ray scattering
Trimers
X-ray scattering
Zinc
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Summary:Controlled self‐assembly (SA) of proteins offers the possibility to tune their properties or to create new materials. Herein, we present the synthesis of a modified human insulin (HI) with two distinct metal‐ion binding sites, one native, the other abiotic, enabling hierarchical SA through coordination with two different metal ions. Selective attachment of an abiotic 2,2′‐bipyridine (bipy) ligand to HI, yielding HI–bipy, enabled ZnII‐binding hexamers to SA into trimers of hexamers, [[HI–bipy]6]3, driven by octahedral coordination to a FeII ion. The structures were studied in solution by small‐angle X‐ray scattering and on surfaces with AFM. The abiotic metal ligand had a higher affinity for FeII than ZnII ions, enabling control of the hexamer formation with ZnII and the formation of trimers of hexamers with FeII ions. This precise control of protein SA to give oligomers of oligomers provides nanoscale structures with potential applications in nanomedicine. Durchstrukturiert: Metallionen vermitteln die Bildung von definierten 18‐meren Insulin‐Nanoaggregaten aus modifizierten Humaninsulin‐Molekülen (schwarze Dreiecke im Schema). Die Bindung an ZnII (grüne Kreise) über einen abiotischen 2,2′‐Bipyridin‐Ligand (rote Ellipse) führt zu Hexameren, die wiederum durch Koordination an ein FeII‐Ion (blauer Kreis) trimerisieren.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201509088