A Novel Method of High Sensitive Determination of Prednisolone on Renewable Mercury Film Silver Based Electrode

Fast and sensitive method of prednisolone determination at renewable mercury film electrode using differential pulse adsorptive stripping voltammetry (DPAdSV) was successfully developed. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and sup...

Full description

Saved in:
Bibliographic Details
Published in:Electroanalysis (New York, N.Y.) N.Y.), 2016-02, Vol.28 (2), p.394-400
Main Authors: Smajdor, Joanna, Piech, Robert, Paczosa-Bator, Beata
Format: Article
Language:English
Subjects:
Composition effects
Concentration (composition)
Electrodes
Electrolytes
mercury film electrode
Prednisolone
Reproducibility
Simulation
steroid determination
Stripping
voltammetry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fast and sensitive method of prednisolone determination at renewable mercury film electrode using differential pulse adsorptive stripping voltammetry (DPAdSV) was successfully developed. The effects of various factors such as: preconcentration potential and time, pulse height, step potential and supporting electrolyte composition were optimized. A linear voltammetric response for analyte was obtained in the concentration range from 0.05 µmol L−1 (18 µg L−1) to 2.25 µmol L−1 (0.81 mg L−1), with a detection limit of 0.01 µmol L−1 for preconcentration time of 20 s. Repeatability of method was determined as RSD % for prednisolone concentration of 0.04 µmol L−1 as 1.6 % (n=9). The proposed method was successfully applied and validated by recovery parameter of prednisolone in simulated sample and pharmaceutical product.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201500262