High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry

In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2016-03, Vol.408 (7), p.1909-1916
Main Authors: Jagannadh, Veerendra Kalyan, Bhat, Bindu Prabhath, Nirupa Julius, Lourdes Albina, Gorthi, Sai Siva
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Analyzers
Automation
Biochemistry
Biomimetics
Blood
Blood cells
cameras
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
cost effectiveness
Counting
Diagnostic systems
diagnostic techniques
Equipment Design
Erythrocyte Count - economics
Erythrocyte Count - instrumentation
Flow cytometry
Flow Cytometry - economics
Flow Cytometry - instrumentation
Food Science
Geometry
Hematology
High-Throughput Screening Assays - economics
High-Throughput Screening Assays - instrumentation
Humans
Image retrieval
Laboratory Medicine
Mathematical analysis
Microfluidic Analytical Techniques - economics
Microfluidic Analytical Techniques - instrumentation
Microfluidics
Microscopy
Microscopy - economics
Microscopy - instrumentation
Monitoring/Environmental Analysis
Optics
Research Paper
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Description
Summary:In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-015-9301-2