Aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated Bacillus cereus GIGAN2 and identification of transformation intermediates

• Published in: Bioresource technology 2015-01, Vol.175, p.563-568
• Main Authors: Liang, Zhishu, An, Taicheng, Li, Guiying, Zhang, Zhengyong
• Format: Article
• Language: English
• Subjects: Aerobiosis; Bacillus cereus; Bacillus cereus - genetics; Bacillus cereus - isolation & purification; Bacillus cereus - metabolism; Biodegradation; Biodegradation, Environmental; Biotechnology - methods; Carbon; China; Dimethyl; Disulfides; Disulfides - metabolism; Gas Chromatography-Mass Spectrometry; Half-Life; Hydrogen-Ion Concentration; Kinetics; Odorants; Sewage - microbiology; Spectrometry; Temperature; Transformations; Water - chemistry; Water - metabolism
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.11.002

A novel, flagellated, rod-shape, Gram-positive facultative aerobe, was isolated and identified as Bacillus cereus GIGAN2. It can effectively remove model odorous organics dimethyl disulfide (DMDS) in aqueous solution under aerobic conditions. Initial concentration, pH value and temperature played important role in DMDS biodegradation, and up to 100% of 10mgL(-1) of DMDS could be removed within 96h under the optimum conditions (30°C, pH 7.0 and 200rpm) with a maximum biodegradation rate constant of 0.0330h(-1) and minimum half-life of 21.0h, respectively. Three main intermediates were identified using gas chromatography-mass spectrometry during this biodegradation process. Further, a reaction scheme is also proposed to explain the possible DMDS biodegradation mechanism by GIGAN2 based on the above-identified intermediates. Overall, this is the first report to demonstrate a newly isolated strain using high concentrated DMDS as the sole carbon and energy source with high efficiency.