Orbitide Composition of the Flax Core Collection (FCC)

The flax (Linum usitatissimum L.) core collection (FCC) was regenerated in Saskatoon, Saskatchewan and Morden, Manitoba in 2009. Seed orbitide content and composition from successfully propagated plants of 391 accessions were analyzed using high-throughput analyses employing high-performance liquid...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2016-06, Vol.64 (25), p.5197-5206
Main Authors: Burnett, Peta-Gaye Gillian, Olivia, Clara Marisa, Okinyo-Owiti, Denis Paskal, Reaney, Martin John Tarsisius
Format: Article
Language:English
Subjects:
Botany - organization & administration
Canada
Chromatography, High Pressure Liquid
Databases, Factual
Flax - chemistry
Flax - classification
Mass Spectrometry
Molecular Structure
Plant Extracts - chemistry
Seeds - chemistry
Seeds - classification
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Summary:The flax (Linum usitatissimum L.) core collection (FCC) was regenerated in Saskatoon, Saskatchewan and Morden, Manitoba in 2009. Seed orbitide content and composition from successfully propagated plants of 391 accessions were analyzed using high-throughput analyses employing high-performance liquid chromatography (HPLC) with reverse-phase monolithic HPLC columns and diode array detection (HPLC–DAD). Seed from plants regenerated in Morden had comparatively higher orbitide content than those grown in Saskatoon. Concentrations of orbitides encoded by contig AFSQ01016651.1 (1, 3, and 8) were higher than those encoded by AFSQ01025165.1 (6, 13, and 17) for most accessions in both locations. The cultivar ‘Primus’ from Poland and an unnamed accession (CN 101580 of unknown origin) exhibited the highest ratio of sum of [1,3,8] to a sum of [6,13,17]. Conversely, the lowest orbitide concentrations and ratio of [1,3,8] to [6,13,17] were observed in cultivars ‘Hollandia’ and ‘Z 11637’, both from The Netherlands. Orbitide expression did not correlate with flax morphological and other chemical traits.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.6b02035