The light-responsive promoter of cyanobacterial ORF76 gene overlaps with the htpG terminator

A Synechococcus PCC7942 gene, encoding 76 amino acids of an unknown protein (designated ORF76), is located at the same orientation and downstream of the htpG gene. The processed site of ORF76 transcripts is located at position +39 with respect to the true transcription initiation site. The non- E. c...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-06, Vol.235 (2), p.341-347
Main Authors: Plansangkate, Prae, Fa-aroonsawat, Sirirat, Panyim, Sakol, Chungjatupornchai, Wipa
Format: Article
Language:English
Subjects:
Cyanobacteria
htpG gene
Light-responsive promoter
Promoter–terminator overlap
Synechococcus
β-glucuronidase
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Summary:A Synechococcus PCC7942 gene, encoding 76 amino acids of an unknown protein (designated ORF76), is located at the same orientation and downstream of the htpG gene. The processed site of ORF76 transcripts is located at position +39 with respect to the true transcription initiation site. The non- E. coliσ 70-like basal promoter of ORF76 (−51 to −9) is controlled by three cis-acting elements: positively acting element (−160 to −86), negative regulatory element (−86 to −51) and light-responsive element (−51 to +63) that, together, respond to high light induction at transcriptional level. The ORF76 gene is expressed as monocistronic transcript. The promoter elements of ORF76 gene overlap with the coding sequence and 3 ′ end formation signal of htpG gene.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.05.012