Radiation hybrid map assignments of 11 ESTs obtained from a 28-day-old swine embryo cDNA library to the IMpRH map

• Published in: Animal genetics 2004-06, Vol.35 (3), p.227-229
• Main Authors: Sarker, N, Kiuchi, S, Yasue, H, Mitsuhashi, T
• Format: Article
• Language: English
• Subjects: Animals; chromosome mapping; comparative chromosome mapping; complementary DNA; DNA libraries; DNA Primers; embryo (animal); Expressed Sequence Tags; Gene Library; Humans; molecular sequence data; nucleic acid hybridization; nucleotide sequences; physical chromosome mapping; porcine; Radiation Hybrid Mapping; sequence analysis; Sequence Analysis, DNA; Sus scrofa; Sus scrofa - genetics; swine
• ISSN: 0268-9146; 1365-2052
• DOI: 10.1111/j.1365-2052.2004.01132.x

Summary In order to improve the map resolution and to locate more genes on the porcine radiation hybrid map, expressed sequence tags (ESTs) were isolated from a 28‐day‐old normal pig embryo cDNA library. The ESTs were sequenced from the 5′‐end and similarities were checked with sequences registered in the NCBI DNA database (http://www.ncbi.nlm.nih.gov/blast/). The ESTs sequences which have high identity scores (>80%) against human genes or ESTs were further sequenced from the 3′ untranslated region. The ESTs which were sequenced successfully were used to design primers for PCR analysis of the radiation hybrid panel. Eleven ESTs were physically mapped to porcine chromosomes 2, 4, 8, 10, 13, 14 and X. The localizations are in agreement with the comparative mapping data between human and pig. The results will provide unique information to the comparative map of human and pig.