The 3 super() termini of small RNAs in Trypanosoma brucei

Post-transcriptional 3 super() end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3 super() ends of mature RNAs is essential for defining 3 super()-processing activities. We have mapped the mature 3 super() ends of Spliced Leader RNA, the U1, U2, U4, U5...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-07, Vol.236 (1), p.73-78
Main Authors: Hitchcock, R A, Zeiner, G M, Sturm, N R, Campbell, DA
Format: Article
Language:English
Subjects:
Trypanosoma brucei
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Summary:Post-transcriptional 3 super() end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3 super() ends of mature RNAs is essential for defining 3 super()-processing activities. We have mapped the mature 3 super() ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear RNAs, the U3 small nucleolar RNA, and the 5S and 5.8S ribosomal RNAs by ligation-mediated PCR in Trypanosoma brucei. With the exception of U5, two classes of 3 super() ends were observed: flush with the base of a stem-loop structure, and 1-2 nt extended from a stem-loop. Multiple mechanisms and structural features are likely to influence 3 super() end maturation of RNAs.
ISSN:0378-1097
DOI:10.1016/j.femsle.2004.05.020