The use of sonication treatment to decellularize aortic tissues for preparation of bioscaffolds

A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of so...

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Bibliographic Details
Published in:Journal of biomaterials applications 2014-07, Vol.29 (1), p.130-141
Main Authors: Azhim, A, Syazwani, N, Morimoto, Y, Furukawa, KS, Ushida, T
Format: Article
Language:English
Subjects:
Animals
Aorta, Thoracic - cytology
Aorta, Thoracic - metabolism
Biocompatible Materials
Biomaterials
Biomechanical Phenomena
Biomechanics
Biomedical materials
Cell Separation
Detergents
DNA - isolation & purification
Extracellular Matrix - chemistry
Implantation
Materials Testing
Rats
Rats, Wistar
Rinsing
Scaffolds
Sodium Dodecyl Sulfate
Sonication - instrumentation
Sonication - methods
Surgical implants
Sus scrofa
Tissue Engineering - methods
Tissue Scaffolds
Transplantation, Heterologous
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Summary:A novel decellularization method using sonication treatment is described. Sonication treatment is the combination of physical and chemical agents. These methods will disrupt cell membrane and release cell contents to external environments. The cell removal was facilitated by subsequent rinsing of sodium dodecyl sulfate detergents. Sonication treatment is used in the preparation of complete decellularized bioscaffolds. The aim of this study is to confirm the usefulness of sonication treatment for preparation of biological scaffolds. In this study, samples of aortic tissues are decellularized by sonication treatment at frequency of 170 kHz in 0.1% and 2% sodium dodecyl sulfate detergents for 10-h treatment time. The relation between decellularization and sonication parameters such as dissolved oxygen concentration, conductivity, and pH is investigated. Histological analysis and biomechanical testing is performed to evaluate cell removal efficiency as well as changes in biomechanical properties. Minimal inflammation response elicit by bioscaffolds is confirmed by xenogeneic implantation and immunohistochemistry. Sonication treatment is able to produce complete decellularized tissue suggesting that these treatments could be applied widely as one of the decellularization method.
ISSN:0885-3282
1530-8022
DOI:10.1177/0885328213517579