In vitro antimicrobial activity and biocompatibility of propolis containing nanohydroxyapatite

The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as we...

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Bibliographic Details
Published in:Biomedical materials (Bristol) 2015-04, Vol.10 (2), p.025004-25011
Main Authors: Grenho, L, Barros, J, Ferreira, C, Santos, V R, Monteiro, F J, Ferraz, M P, Cortes, M E
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Animals
Anti-Infective Agents - isolation & purification
Anti-Infective Agents - pharmacology
antibacterial activity
Bacteria
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
Biofilms
Biofilms - drug effects
Biofilms - growth & development
Biomaterials
Biomedical materials
cytocompatibility
Durapatite - chemistry
Fibroblasts - drug effects
Fibroblasts - metabolism
Humans
In Vitro Techniques
Materials Testing
Mice
nanohydroxyapatite
Nanostructure
Nanostructures - chemistry
Propolis
Propolis - isolation & purification
Propolis - pharmacology
Prosthesis-Related Infections - microbiology
Prosthesis-Related Infections - prevention & control
Reduction
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - physiology
Surface chemistry
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Summary:The high number of biomaterial associated infections demands new strategies to prevent this problem. In this study the suitability of nanohydroxyapatite (nanoHA)-based surfaces containing two Brazilian extracts of propolis (green and red ones) to prevent bacterial growth and biofilm formation, as well as its non-cytotoxic nature, was investigated. Optical density, colony forming units and MTT reduction assay were used to assess the materials' antibacterial activity against planktonic and sessile growth of Staphylococcus aureus. NanoHA matrix was able to absorb both types of propolis and the obtained results revealed the antibacterial effectiveness of the novel materials expressed as the reduction of bacterial growth and biofilm formation ability. Additionally, cell culture tests showed the growth of fibroblasts with high metabolic activity and without membrane damage. Therefore, these nanoHA-based surfaces containing natural products deriving from bees may be a promising bioactive biomaterial to be further studied with the aim of application to orthopaedic or dental devices.
ISSN:1748-6041
1748-605X
1748-605X
DOI:10.1088/1748-6041/10/2/025004