Nanoactivator mediated modifications in thermostable amylase from Bacillus licheniformis

Gram-positive rod-shaped thermophilic bacteria were isolated using samples collected from terrestrial natural thermal spring located at Unkeshwar (Longitude 78.22 degree East to 78.34 degree East, Latitude 19 degree 34' North to 19 degree 40' North), District Nanded, Maharashtra State, Ind...

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Bibliographic Details
Published in:Indian journal of biochemistry & biophysics 2012-12, Vol.49 (6), p.468-471
Main Authors: Khairnar, Rajendra S, Mahabole, Megha P, Pathak, Anupama P
Format: Article
Language:English
Subjects:
Amylases - chemistry
Amylases - metabolism
Bacillus - enzymology
Bacillus - isolation & purification
Bacillus licheniformis
Calcium - chemistry
Catalysis
Freshwater
Hot Temperature
Hydrogen-Ion Concentration
Nanostructures
Nanotechnology
Phosphates - chemistry
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Summary:Gram-positive rod-shaped thermophilic bacteria were isolated using samples collected from terrestrial natural thermal spring located at Unkeshwar (Longitude 78.22 degree East to 78.34 degree East, Latitude 19 degree 34' North to 19 degree 40' North), District Nanded, Maharashtra State, India. The isolates were then cultivated using selective media and identified using culture-dependent techniques. One prominent isolate (UN1) exhibited high temperature stability and remarkable amylase production and was identified as Bacillus licheniformis. Amylase production was carried out in starch media and the enzyme was partially purified and characterized for optimization of pH and temperature. Amylolytic activity of the enzyme was determined. Nanoactivator-mediated modifications were carried out to enhance amylolytic activity of the partially purified amylase. Three-fold increase in catalytic efficiency of amylase was obtained after modification.
ISSN:0301-1208