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Molecular analysis of hemagglutinin-1 fragment of avian influenza H5N1 viruses isolated from chicken farms in Indonesia from 2008 to 2010

•Sequence data from commercial poultry, which are not publicly available.•No significant positive selection of residues was detected.•Complete protectivity is gained in challenge with homologous strains.•Protectivity ranges from 40 to 100% in challenge with heterologous strains.•The implication for...

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Bibliographic Details
Published in:Veterinary microbiology 2016-04, Vol.186, p.52-58
Main Authors: Mahardika, Gusti N., Jonas, Melina, Murwijati, Theresia, Fitria, Nur, Suartha, I Nyoman, Suartini, I Gusti A.A., Wibawan, I Wayan Teguh
Format: Article
Language:English
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Summary:•Sequence data from commercial poultry, which are not publicly available.•No significant positive selection of residues was detected.•Complete protectivity is gained in challenge with homologous strains.•Protectivity ranges from 40 to 100% in challenge with heterologous strains.•The implication for vaccination strategies is discussed. Highly pathogenic avian influenza virus of subtype H5N1 (AIV-H5N1) has been circulating in Indonesia since 2003. To understand the genetic diversity of these viruses, and to predict vaccine efficacy, the hemaglutinin-1 (HA-1) fragment of viruses isolated from chicken farms in Indonesia from 2008 to 2010 was sequenced and analyzed. The effects of these molecular changes were investigated in challenge experiments and HI assays of homologous and heterologous strains. Molecular analysis showed that these AIV-H5N1 isolates had evolved into three distinct sub-lineages from an ancestor circulating since 2003. Although no significant positive selection of residues was detected, 12 negatively selected sites were identified (p
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2016.02.023