Molecular mechanism of endocrine system impairment by 17α-methyltestosterone in gynogenic Pengze crucian carp offspring

• Published in: Ecotoxicology and environmental safety 2016-06, Vol.128, p.143-152
• Main Authors: Zheng, Yao, Chen, Jiazhang, Liu, Yan, Gao, Jiancao, Yang, Yanping, Zhang, Yingying, Bing, Xuwen, Gao, Zexia, Liang, Hongwei, Wang, Zaizhao
• Format: Article
• Language: English
• Subjects: 17α-methyltestosterone; Androgens - toxicity; Animals; Aromatase - metabolism; Brain - drug effects; Brain - metabolism; Carps - genetics; Carps - growth & development; Carps - metabolism; Endocrine Disruptors - toxicity; Endocrine System - drug effects; Endocrine System - metabolism; Estradiol - genetics; Estradiol - metabolism; Estrogen signalling; Female; Freshwater; Gene Expression Regulation, Developmental - drug effects; Gynogenic Pengze crucian carp; Liver - drug effects; Liver - metabolism; Methyltestosterone - toxicity; Ovum - drug effects; Ovum - metabolism; Receptors, Steroid - genetics; Receptors, Steroid - metabolism; Sex Characteristics; Steroid hormones; Steroidogenesis; Testosterone - genetics; Testosterone - metabolism; Vitellogenesis; Vitellogenesis - drug effects; Vitellogenesis - genetics; Vitellogenins - genetics; Vitellogenins - metabolism
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2015.11.034

The effects of synthetic androgen 17α-methyltestosterone (MT) on endocrine impairment were examined in crucian carp. Immature 7-month old mono-female Pengze crucian carp (Pcc) F2 offspring were exposed to 50 and 100μg/L of MT (week 2, 4, and 8). Gonadosomatic index, hepatosomatic index and intestine weight altered considerably and oocyte development was repressed. In the treatment groups, ovarian 11-ketotestosterone decreased, whereas 17β-estradiol and testosterone increased, and ovarian aromatase activities increased at week 4. However, in the brain tissue, those values significantly decreased. Quantitative RT-PCR analysis demonstrated changes in steroid receptor genes and upregulation of steroidogenic genes (Pcc-3bhsd, Pcc-11bhsd2 Pcc-cyp11a1), while the other three steroidogenic genes (Pcc-cyp17a1, Pcc-cyp19a1a and Pcc-star) decreased from week 4 to week 8. Ovarian, hepatic Pcc-vtg B and vitellogenin concentration increased in both 50 and 100μg/L of MT exposure groups. This study adds further information regarding the effects of androgens on the development of previtellogenic oocytes, which suggests that MT could directly target estrogen signaling pathway, or indirectly affect steroidogenesis and vitellogenesis. •Partial cDNAs of five steroidogenic genes of gynogenic fish were obtained.•The steroid hormones (11-KT, T and E2) balance was disrupted following MT exposures.•Ovarian and hepatic vitellogenesis showed significant increments.•Some morphological indicators were altered and oocyte cell development was repressed.