Retrospective diagnosis of two rabies cases in humans by high throughput sequencing

Highlights • Two cases of rabies among humans were detected using a broad-range PCR analysis followed by high throughput sequencing. • Diagnoses were subsequently confirmed using a fluorescent antibody test, an enzyme-linked immunosorbent assay (ELISA) and a mouse inoculation test. • Two strains of...

Full description

Saved in:
Bibliographic Details
Published in:Journal of clinical virology 2016-05, Vol.78, p.74-81
Main Authors: Dedkov, V.G, Lukashev, A.N, Deviatkin, A.A, Kuleshov, K.V, Safonova, M.V, Poleshchuk, E.M, Drexler, J.F, Shipulin, G.A
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Autopsy
Broad-range PCR
Child, Preschool
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique
High throughput sequencing
High-Throughput Nucleotide Sequencing
Humans
Infectious Disease
Male
Mice
Middle Aged
Polymerase Chain Reaction
Rabies - diagnosis
Rabies - virology
Rabies virus
Rabies virus - genetics
Rabies virus - isolation & purification
Retrospective Studies
Russia
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Highlights • Two cases of rabies among humans were detected using a broad-range PCR analysis followed by high throughput sequencing. • Diagnoses were subsequently confirmed using a fluorescent antibody test, an enzyme-linked immunosorbent assay (ELISA) and a mouse inoculation test. • Two strains of rabies virus were isolated and characterized using virological methods. • The entire genome of each strain was sequenced.
ISSN:1386-6532
1873-5967
DOI:10.1016/j.jcv.2016.03.012