Increased Interleukin-4 Production by CD8 and gamma delta T Cells in Health-Care Workers Is Associated with the Subsequent Development of Active Tuberculosis

• Published in: The Journal of infectious diseases 2004-08, Vol.190 (4), p.756-766
• Main Authors: Ordway, D J, Costa, L, Martins, M, Silveira, H, Amaral, L, Arroz, MJ, Ventura, F A, Dockrell, H M
• Format: Article
• Language: English
• Subjects: Mycobacterium tuberculosis
• ISSN: 0022-1899
• DOI: 10.1086/422532

We evaluated immune responses to Mycobacterium tuberculosis in 10 health-care workers (HCWs) and 10 non-HCWs and correlated their immune status with the development of active tuberculosis (TB). Twenty individuals were randomly recruited, tested, and monitored longitudinally for TB presentation. Peripheral blood mononuclear cells (PBMCs) from donors were stimulated with M. tuberculosis and tested for cell proliferation and the production of interferon (IFN)- gamma , interleukin (IL)-5, and IL-4, by use of enzyme-linked immunosorbent or flow-cytometric assays. HCWs had higher levels of cell proliferation (24,258 cpm) and IFN- gamma (6373 pg/mL) to M. tuberculosis than did non-HCWs (cell proliferation, 11,462 cpm; IFN- gamma , 3228 pg/mL). Six of 10 HCWs showed increased median percentages of CD8 super(+)IL-4 super(+) (4.7%) and gamma delta super(+)IL-4 super(+) (2.3%) T cells and progressed to active TB. HCWs who remained healthy showed increased median percentages of CD8 super(+)IFN- gamma super(+) (25.0%) and gamma delta super(+)IFN- gamma super(+) (8.0%) and lower percentages of CD8 super(+)IL-4 super(+) (0.05%) and gamma delta super(+)IL-4 super(+) (0.03%) T cells.