Expression of chicken ovalbumin upstream promoter-transcription factor II enhances invasiveness of human lung carcinoma cells

Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) plays an essential role in angiogenesis and development. It is differentially expressed in tumor cell lines, but its role in carcinogenesis is largely unknown. We demonstrate here that noninvasive human lung cancer cells become...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2004-08, Vol.64 (15), p.5097-5105
Main Authors: NAVAB, Roya, GONZALEZ-SANTOS, Juana Maria, JOHNSTON, Michael R, JIANG LIU, BRODT, Pnina, TSAO, Ming-Sound, HU, Jim
Format: Article
Language:English
Subjects:
Actins - metabolism
Antineoplastic agents
Biological and medical sciences
Blotting, Western
COUP Transcription Factor II
COUP Transcription Factors
DNA-Binding Proteins - genetics
Focal Adhesion Kinase 1
Focal Adhesion Protein-Tyrosine Kinases
Gene Expression Regulation, Neoplastic - physiology
Humans
Immunoblotting
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Matrix Metalloproteinase 2 - metabolism
Medical sciences
Neoplasm Invasiveness
Pharmacology. Drug treatments
Phosphorylation
Plasmids
Protein-Tyrosine Kinases - metabolism
Receptors, Steroid
Transcription Factors - genetics
Transfection
Tumor Cells, Cultured
Tumors
Up-Regulation
Urokinase-Type Plasminogen Activator - metabolism
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Summary:Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) plays an essential role in angiogenesis and development. It is differentially expressed in tumor cell lines, but its role in carcinogenesis is largely unknown. We demonstrate here that noninvasive human lung cancer cells become invasive when COUP-TFII was expressed. The expression of extracellular matrix degrading proteinases, such as matrix metalloproteinase 2 and urokinase-type plasminogen activator, was up-regulated in these cells. This finding was confirmed by transduction of different human lung cancer cell lines with COUP-TFII protein and also by using antisense expression. We observed disorganization of actin filaments and focal adhesion kinase phosphorylation in COUP-TFII-transfected human lung cancer cells in addition to the increase in extracellular metalloproteinase activity. These results suggest that COUP-TFII may be considered as a new target for anticancer therapies.
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.CAN-03-1185