Liquid chromatography/photodiode array detection for determination of strychnine in blood: a fatal case report

An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1ml of sample. The strychnine was isolated from blood using a liquid–liquid extraction procedure and chloroquine as...

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Bibliographic Details
Published in:Forensic science international 2004-04, Vol.141 (1), p.17-21
Main Authors: Duverneuil, Charlotte, Grandmaison, Geoffroy Lorin de la, Mazancourt, Philippe de, Alvarez, Jean-Claude
Format: Article
Language:English
Subjects:
Adult
Analysis
Analytical chemistry
Antimalarials
Beer
Biological and medical sciences
Blood
Blood banks
Calibration
Chloroquine
Chromatography
Chromatography, High Pressure Liquid - methods
Criminal investigations
Forensic Medicine - methods
Forensic sciences
Forensic toxicology
Gas Chromatography-Mass Spectrometry
High performance liquid chromatography
HPLC
Humans
Indicators and Reagents
Investigative techniques, diagnostic techniques (general aspects)
Male
Mass spectrometry
Medical sciences
Neurology
Photodiode-array
Plasma
Poisoning
Poisoning - diagnosis
Poisons - blood
Quality control
Strychnine
Strychnine - blood
Strychnine - poisoning
Vascular diseases and vascular malformations of the nervous system
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Summary:An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1ml of sample. The strychnine was isolated from blood using a liquid–liquid extraction procedure and chloroquine as an internal standard. The limits of detection (LOD) and quantification were 0.06 and 0.5mg/l, respectively. The recovery was 94% and the coefficients of variation (CV) ranged from 5.9 to 10.8%. A fatal case of strychnine poisoning is presented, with a lethal blood concentration of 25mg/l.
ISSN:0379-0738
1872-6283
DOI:10.1016/j.forsciint.2003.12.010