Sanguinate's effect on pial arterioles in healthy rats and cerebral oxygen tension after controlled cortical impact

• Published in: Microvascular research 2016-09, Vol.107, p.83-90
• Main Authors: Mullah, Saad H., Abutarboush, Rania, Moon-Massat, Paula F., Saha, Biswajit K., Haque, Ashraful, Walker, Peter B., Auker, Charles R., Arnaud, Francoise G., McCarron, Richard M., Scultetus, Anke H.
• Format: Article
• Language: English
• Subjects: Animals; Arterial Pressure - drug effects; Arterioles - drug effects; Arterioles - metabolism; Arterioles - physiopathology; Brain Injuries, Traumatic - drug therapy; Brain Injuries, Traumatic - metabolism; Brain Injuries, Traumatic - physiopathology; Carboxyhemoglobin - analogs & derivatives; Carboxyhemoglobin - pharmacology; Carboxyhemoglobin - toxicity; Cerebral oxygenation; Cerebrovascular Circulation - drug effects; Disease Models, Animal; Hemoglobin-based oxygen carrier; Hydroxyethyl Starch Derivatives - pharmacology; Intravital microscopy; Male; Microcirculation; Microcirculation - drug effects; Oxygen - metabolism; Oxygen Consumption - drug effects; Oxygen therapeutic; Phosphorescence quenching method; Pia Mater - blood supply; Plasma Substitutes - pharmacology; Plasma Substitutes - toxicity; Polyethylene glycol-conjugated carboxyhemoglobin; Polyethylene Glycols - pharmacology; Polyethylene Glycols - toxicity; Rats, Sprague-Dawley; Time Factors; Traumatic brain injury; Vasoconstriction - drug effects
• ISSN: 0026-2862; 1095-9319
• DOI: 10.1016/j.mvr.2016.06.001

Sanguinate, a polyethylene glycol-conjugated carboxyhemoglobin, was investigated for cerebral vasoactivity in healthy male Sprague-Dawley rats (Study 1) and for its ability to increase brain tissue oxygen pressure (PbtO2) after controlled cortical impact (CCI) — traumatic brain injury (TBI) (Study 2). In both studies ketamine-acepromazine anesthetized rats were ventilated with 40% O2. In Study 1, a cranial window was used to measure the diameters of medium — (50–100μm) and small-sized (