A novel glucose biosensor platform based on Ag@AuNPs modified graphene oxide nanocomposite and SERS application

• Published in: Journal of colloid and interface science 2013-09, Vol.406, p.231-237
• Main Authors: Gupta, Vinod Kumar, Atar, Necip, Yola, Mehmet Lütfi, Eryılmaz, Merve, Torul, Hilal, Tamer, Uğur, Boyacı, İsmail Hakkı, Üstündağ, Zafer
• Format: Article
• Language: English
• Subjects: Ag@AuNPs; Biosensing Techniques - methods; biosensors; blood glucose; Blood Glucose - analysis; Boronic Acids - chemistry; Characterization; Chemistry; Colloidal state and disperse state; detection limit; Enzymes, Immobilized - metabolism; Exact sciences and technology; General and physical chemistry; glucose; Glucose biosensor; glucose oxidase; Glucose Oxidase - metabolism; Gold - chemistry; Graphene oxide; Graphite - chemistry; Humans; nanocomposites; Nanocomposites - chemistry; nanomaterials; Photoelectron Spectroscopy; Physical and chemical studies. Granulometry. Electrokinetic phenomena; SERS; Silver - chemistry; Spectrum Analysis, Raman; Sulfhydryl Compounds - chemistry; transmission electron microscopy; X-ray diffraction; X-ray photoelectron spectroscopy
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2013.06.007

[Display omitted] •We prepared mercaptophenyl boronic acid terminated Ag@AuNPs/graphene oxide.•The prepared nanocomposites were characterized by using various techniques.•The nanocomposite shows excellent SERS activity.•The biosensor was applied for the determination of glucose in blood samples. This study represents a novel template demonstration of a glucose biosensor based on mercaptophenyl boronic acid (MBA) terminated Ag@AuNPs/graphene oxide (Ag@AuNPs–GO) nanomaterials. The nanocomposites were characterized by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD) method. The TEM image shows that Ag@AuNPs in the nanocomposite is in the range of diameters of 10–20nm. The nanocomposite was used for the determination of glucose through the complexation between boronic acid and diol groups of glucose. Thus, a novel glucose biosensor was further fabricated by immobilizing glucose oxidase (GOD) into MBA terminated Ag@AuNPs–GO nanocomposite film (MBA–Ag@AuNPs–GO). The linearity range of glucose was obtained as 2–6mM with detection limit of 0.33mM. The developed biosensor was also applied successfully for the determination of glucose in blood samples. The concentration value of glucose in blood samples was calculated to be 1.97±0.002mM from measurements repeated for six times.