Induction of cytotoxicity, oxidative stress and genotoxicity by root filling pastes used in primary teeth

Aim To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes. Methodology Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the...

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Bibliographic Details
Published in:International endodontic journal 2016-08, Vol.49 (8), p.737-745
Main Authors: Pires, C. W., Botton, G., Cadoná, F. C., Machado, A. K., Azzolin, V. F., da Cruz, I. B. M., Sagrillo, M. R., Praetzel, J. R.
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
biocompatibility
calcium hydroxide
Cattle
deciduous tooth
Dentistry
DNA - drug effects
DNA Damage
Humans
Hydrocarbons, Iodinated - pharmacology
iodoform
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Materials Testing
Oxidative Stress - drug effects
pulpectomy
Reactive Oxygen Species - metabolism
root canal filling materials
Root Canal Filling Materials - pharmacology
Statistics, Nonparametric
Tooth, Deciduous
Toxicity Tests
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Summary:Aim To evaluate the cytotoxicity, oxidative stress and genotoxicity in vitro of four iodoform pastes and three calcium hydroxide pastes. Methodology Peripheral blood mononuclear cells (PBMCs) and pure calf thymus DNA (dsDNA) were exposed to extracts of the pastes. Cytotoxicity was assessed with the MTT assay. Generation of reactive oxygen species (ROS) was evaluated using a DCFH‐DA assay, and lipid peroxidation was evaluated using a TBARS assay. Genotoxicity was evaluated using the alkaline comet assay and Genomodifier capacity assay (GEMO). All tests were performed after 24 h and 72 h of cell exposure, except GEMO. After performing the Kolmogorov–Smirnov test, data were analysed by Kruskal–Wallis and Dunn's post‐tests, and anova with Dunnett's post‐test, with a significance level established at P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.12502