Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine

Aim To evaluate the capacity of commonly used root canal irrigants to induce cytotoxic effects, when applied singly or in combination. The hypothesis tested was that the irrigants were less cytotoxic when applied in combination than independently. Methodology MRC5 cells were grown as monolayer cultu...

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Bibliographic Details
Published in:International endodontic journal 2016-08, Vol.49 (8), p.764-773
Main Authors: Vouzara, T., Koulaouzidou, E., Ziouti, F., Economides, N.
Format: Article
Language:English
Subjects:
Analysis of Variance
Anti-Infective Agents, Local - pharmacology
Cell Line
chlorhexidine
Chlorhexidine - pharmacology
cytotoxicity
Dentistry
Edetic Acid - pharmacology
ethylenediaminetetraacetic acid
Humans
irrigant combinations
Root Canal Irrigants - pharmacology
sodium hypochlorite
Sodium Hypochlorite - pharmacology
Toxicity Tests
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Summary:Aim To evaluate the capacity of commonly used root canal irrigants to induce cytotoxic effects, when applied singly or in combination. The hypothesis tested was that the irrigants were less cytotoxic when applied in combination than independently. Methodology MRC5 cells were grown as monolayer cultures at 37 °C in an atmosphere containing 5% CO2 in air and 100% relative humidity. Cells were exposed to sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), chlorhexidine (CHX) and their combinations (NaOCl/EDTA, NaOCl/CHX, EDTA/CHX) in serial dilutions. Growth medium was Dulbecco's modified Eagle's medium supplemented with 10% foetal bovine serum and antibiotics and was used as control. The effect on cell survival was estimated after 6 and 24 h of exposure by means of the sulforhodamine B assay, in reference to controls. Dose–response curves were plotted, and 50% inhibitory doses (IC50) were subjected to statistical analysis (anova and post hoc comparison test; P 
ISSN:0143-2885
1365-2591
DOI:10.1111/iej.12517